William S. Klug Michael R. Cummings Charlotte A. Spencer Concepts of Genetics Eighth Edition Chapter 10 DNA Structure and Analysis Copyright © 2006 Pearson Prentice Hall, Inc.

The Genetic Material Must Exhibit Four Characteristics Replication Storage of information Expressison of information Variation by mutation

Figure 10-1 Copyright © 2006 Pearson Prentice Hall, Inc.

Until 1944, Observations Favored Protein as the Genetic Material

Figure 10-2 Copyright © 2006 Pearson Prentice Hall, Inc.

Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages Transformation: Early Studies

Figure 10-3 Copyright © 2006 Pearson Prentice Hall, Inc.

Table 10-1 Copyright © 2006 Pearson Prentice Hall, Inc.

Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages Transformation: The Avery, MacLeod, and McCarty Experiment

Figure 10-4 Copyright © 2006 Pearson Prentice Hall, Inc.

Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages The Hershey–Chase Experiment

Figure 10-5 Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 10-6 Copyright © 2006 Pearson Prentice Hall, Inc.

Evidence Favoring DNA as the Genetic Material Was First Obtained during the Study of Bacteria and Bacteriophages Transfection Experiments

Indirect and Direct Evidence Supports the Concept that DNA Is the Genetic Material in Eukaryotes Indirect Evidence: Distribution of DNA Indirect Evidence: Mutagenesis

Table 10-2 Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 10-7 Copyright © 2006 Pearson Prentice Hall, Inc.

Indirect and Direct Evidence Supports the Concept that DNA Is the Genetic Material in Eukaryotes Direct Evidence: Recombinant DNA Studies

RNA Serves as the Genetic Material in Some Viruses

Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure Nucleotides: Building Blocks of Nucleic Acids

Figure 10-9a Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 10-9b Copyright © 2006 Pearson Prentice Hall, Inc.

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Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure Nucleoside Diphosphates and Triphosphates

Figure Copyright © 2006 Pearson Prentice Hall, Inc. (dATP)

Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure Polynucleotides

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Figure 10-12a Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 10-12b Copyright © 2006 Pearson Prentice Hall, Inc.

The Structure of DNA Holds the Key to Understanding Its Function Base Composition Studies X-Ray Diffraction Analysis

Table 10-3 Copyright © 2006 Pearson Prentice Hall, Inc.

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The Structure of DNA Holds the Key to Understanding Its Function The Watson–Crick Model

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Figure 10-14a Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 10-14b Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 10-14c Copyright © 2006 Pearson Prentice Hall, Inc.

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Alternative Forms of DNA Exist

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Figure 10-17a Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 10-17b Copyright © 2006 Pearson Prentice Hall, Inc.

The Structure of RNA Is Chemically Similar to DNA, but Single Stranded

Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Absorption of Ultraviolet Light (UV) Sedimentation Behavior

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Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Denaturation and Renaturation of Nucleic Acids

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Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Molecular Hybridization

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Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Fluorescent in situ Hybridization (FISH)

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Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Reassociation Kinetics and Repetitive DNA

Figure Copyright © 2006 Pearson Prentice Hall, Inc. C/C 0 = 1/1+kC 0 t

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Many Analytical Techniques Have Been Useful during the Investigation of DNA and RNA Electrophoresis of Nucleic Acids

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