EFFECTS OF AND RESISTANCE TO WEAK ACIDS IN SPOILAGE YEASTS.

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Presentation transcript:

EFFECTS OF AND RESISTANCE TO WEAK ACIDS IN SPOILAGE YEASTS

Some weak acids: E 200 sorbic acid mg Kg -1 E 210benzoic acid mg Kg -1 E 220SO mg Kg -1 E 260acetic acid“quantum satis” E 280 propionic acid mg Kg -1

AH A - + H + AH A - + H + neutral cytoplasm acidic environment

EFFECTS OF WEAK ACIDS - Intracellular acidification - Inhibition of glycolytic enzymes - Decrease of fermentation activity - ATP depletion - Growth inhibition - Enhancement of thermal death

- Intracellular acidification, but...

Intracellular acidification S. cerevisiae adaptation energy no adaptation energy no adaptation no energy

- Inhibition of glycolytic enzymes - Decrease of fermentation activity... but...

Specific glucose consumption rate S. cerevisiae

Inhibition of growth ATP is diverted for maintenance purposes...but how much ATP?

S. cerevisiae acetic acid

AH A - + H + AH A - + H + ATP H+H+ A-A- neutral cytoplasm acidic environment ?

glucose Extrusion of benzoic acid S. cerevisiae Adapted cells Non adapted

- Saccharomyces cerevisiae - Zygosaccharomyces bailii - Pichia sp.

Diffusion of benzoic acid Pichia sp. S. cerevisiae Z. bailii non adapted adapted

AH A - + H + AH A - + H + ATP H+H+ A-A- neutral cytoplasm acidic environment conversion Mechanisms of yeast tolerance to preservatives: 1) Reduced permeability; 2) Proton extrusion; 3) Preservative conversion; 4) Preservative extrusion