Effects of Glucose on the Growth Curve of Pseudomonas fluorescens Muhammad Khan & Andrew Passer November 28, 2011.

Slides:

Advertisements

Similar presentations

The Effect of Caffeine on Growth of Pseudomonas fluorescens Author: Eric Huffman Samy Behairy.

Advertisements

Bacterial Growth and Decay By: Karina Vanderbilt, Heidi Pang, & Yina Lor.

Experimental Evolution Ben Callahan Koshlan Meyer-Blackwell Naama Pnina Dekel Hopkins Microbiology Course 2010.

Rate of things via spectrophotometry Aph 162, Winter 2009 Week 2.

Key Area 6 : Growth in Micro-organisms

BIOLOGY What is biology? Biology is the study of life. What is an organism? Each individual living thing. Example: Bacteria, Paramecium, Cat, Rose etc.

Cell Growth Kinetics Introduction

Microbial Growth. Growth of Microbes Increase in number of cells, not cell size One cell becomes colony of millions of cells.

Chapter 3, Section 4 The DNA Connection Monday, November 16, 2009 Pages

METABOLISM. WHAT IS METABOLISM? OUR BODIES GET THE ENERGY THEY NEED FROM FOOD THROUGH METABOLISM THE CHEMICAL REACTIONS IN THE BODY'S CELLS THAT CONVERT.

PHOTOSYNTHESIS AND CARBOHYDRATE PRODUCTION

Biotechnology Use of living organisms and growth curve.

Bacteria are known for their rapid growth, many of the enterics will grow and then divide every 20 minutes under ideal conditions. Some bacteria isolated.

By Connie Lee PI: Stephen Giovannoni Mentor: Amy Carter Department of Microbiology, OSU HHMI Summer Program 2011 Culturing experiments to determine the.

Photosynthesis Part II. Step 2 - Light-Independent Reactions Glucose.

Ecology: We are all connected and interdependent Bonus #3 due 4/30/08.

A chemostat approach to analyze the distribution of metabolic fluxes in wine yeasts during alcoholic fermentation Quirós, M. 1, Martínez-Moreno, R. 1,

Lecture 6: Product Formation Stoichiometry

Chapter 13 Rates of Reaction Dr. S. M. Condren.

Bacterial growth:. Bacterial Growth Curve: The schematic growth curve shown below is associated with simplistic conditions known as a batch culture. It.

The biological catalyst

Chemical Kinetics “Rates of Reactions”. Reaction Rates Average rate: Change of reactant or product concentrations over a specific time interval Initial.

Michelle Anthony Khadija Lawrence. Gram-negative Rod shaped Pathogenic strains day/agriculture/images/e_coli.jpg.

Review of MOCT Questions

Chemistry of Life CellsGeneticsEcologyCell DivisionDNA & RNA

Chapter Living things have cells Multi-cellular (many cells) Uni-cellular (one cell) A cell is a membrane- covered structure containing materials.

Structure of the bacterial cell VI- Bacterial Spore - Definition - Formation - Shape - Importance.

Introduction to Bacteriology

Dr Rita Oladele Dept of Med Micro &Para CMUL/LUTH

Single and Multiple Substrate Kinetics A culture grown in a simple medium including 0.3% wt/vol of glucose; at time t=0 , it is inoculated into.

Effects of salt on growth of P. fluorescens Alan Bohn and Liesel Hannes November 30, 2011 MB495 - Brown.

Metabolites – primary and secondary. Learning Outcomes Compare and contrast the processes of continuous culture and batch culture. Describe the differences.

Diffusion The movement of particles across the cell membrane from an area of high concentration to low concentration Type of passive transport (does not.

Bacterial growth The mathematics of bacterial growth is fairly simple, since each original cell divides to form two new cells, with the loss of the original.

Cell Growth Kinetics -Introduction -Growth patterns and kinetics in batch culture - growth phases - effect of factors: oxygen supply - heat generation.

NAJRAN UNIVERSITY College of Medicine NAJRAN UNIVERSITY College of Medicine Microbiology &Immunology Course Lecture No. 4 Microbiology &Immunology Course.

Microbial Growth refers to increase in number of cells not in size.

Growth Chapter 3. GROWTH CYCLE ● Bacteria reproduce by binary fission, a process by which one parent cell divides to form two progeny cells. ● Because.

Bacterial Growth November 2013.

Bacterial Growth and Reproduction Kelly Spiller East View High School Georgetown TX

Topic: Cells Processes Aim: Describe the life processes that occur in living things. Do Now: Take out yesterday’s reading notes. Phase Changes ISA HW:

Bacterial Nutrition, Metabolism and growth

Ecology: We are all connected and interdependent

Homeostasis and Biochemistry

Bioprocessing presentation

Hyun Suk Park Anh Trinh Carissa Best

MICROBIAL GROWTH CURVE

MIC 303 INDUSTRIAL AND ENVIRONMENTAL MICROBIOLOGY

Factors affecting population growth

Microorganisms & Biotechnology

Objective: 8.L.5.1 –Summarize how food provides the energy and molecules required for building materials, growth, and survival of all organisms.

Characteristics of Life

Today: What happens near the carrying capacity?

Ecology: We are all connected and interdependent

Week 11 Vocab Definitions

Effects of Tetracycline and Kanamycin on Pseudomonas fluorescens

Unit 4: Animal Structure & Function

Cellular Respiration.

Jonathan Knight and Richard Gregory MB 495 November 28, 2011

Characteristics of Living Things

Influence of K+ uptake on S. aureus metabolism.

5 Minute Brainstorm: What is SCIENCE? What is BIOLOGY?

Metabolism and Survival

Cell Structures and Functions (Eukaryotic Cells)

Bacteria are known for their rapid growth, many of the enterics

Unit Environmental Control of Metabolism

5 Minute Brainstorm: What is SCIENCE? What is BIOLOGY?

THE GROWTH ENVIRONMENT.

Presentation transcript:

Effects of Glucose on the Growth Curve of Pseudomonas fluorescens Muhammad Khan & Andrew Passer November 28, 2011

Our Question: What effect does the addition of glucose have on the growth curve of Pseudomonas fluorescens?

Interest in Glucose Experiment 2Experiment 3 ??

Experiment 4 Supports Experiment 3

Is glucose metabolism in P. fluorescens acquired or inherited? We could not “shock” P. fluorescens into increased growth by raising it in a glucose-rich, tryptone-poor environment prior to inoculation.

Calculating "Growth Points"

What Concentration of Glucose Gives the Highest Level of Growth? Average: 3.418% glucose

Conclusion Addition of glucose has a positive effect on the growth of P. fluorescens. Glucose metabolism is an inherited trait for this species. Optimum growth occurs at about 3.4% glucose.

Discussion & Future Directions More time needed for P. fluorescens to grow. Is the prolonged increase in OD actual growth? What are the waste products of the different metabolic pathways (amino acid vs. sugar)? How do the waste products affect the stationary phase?

Any questions?