See using a flow cytometry assay expression of 3 goat poly clonal antibodies to purified viral proteins in both T and B cells but not in T cells from a.

Slides:

Advertisements

Similar presentations

Topics 6&11 Defence against infectious disease cont’d.

Advertisements

In latent infection- retroviral genome is present but is not transcribing viral genome or mRNA for structural proteins. Distinguished by qPCR (DNA) and.

The Immune Response. The Third Line of Defense The third line of defense involves a specific response that is effective against specific pathogens. This.

Viruses Higher Human Biology. Lesson Aims To describe the structure of a virus To examine the process of viral replication.

NK cells kill targets that do not express HLA class I.

 Classification of animal viruses › Taxonomic criteria based on  Genomic structure  DNA or RNA  Single-stranded or double-stranded  Virus particle.

JEOPARDY Back to the Basics Non-Specific Defenses Specific Defenses The Foreign Invasion AIDS $100 $200 $300 $400 $500.

Evidence for the presence of XMRV in 33 PCR Negative US CFS Patients 19/33 Antibodies in the plasma 30/33 Transmissable virus in the plasma 10/33 Protein.

Detection of XMRV/pMuLV Infections in Patients with Chronic Fatigue Syndrome and Healthy Blood Donors Shyh-Ching Lo, Ph.D., M.D. Division of Cellular and.

Chapter 4: Serology Concepts. What is an antigen?  An antigen is any substance that elicits an immune response and is then capable of binding to the.

HIV Structure, Lifecycle, and Replication

11.1 Defense Against Infectious Disease. Process of blood clotting When small vessels burst, blood escapes into your surrounding tissues (often these.

Monoclonal antibodies Hybridoma Technique. Monoclonal antibodies (mAb or moAb) Monoclonal antibodies are:  monospecific antibodies that are identical.

Test 6 Specific cellular immune function assay. Separation of Mononuclear Cells from Human Peripheral BloodSeparation of Mononuclear Cells from Human.

Dr. Peter R. Ganz Director, Centre for Blood and Tissues Evaluation, Health Canada Strategies for Managing Potential Xenotropic Murine Virus Related Virus.

KEY CONCEPT Genetics provides a basis for new medical treatments.

Xenotropic Murine Leukemia Virus Related Virus (XMRV) Informational Presentation BPAC meeting, July 26, 2010 Indira Hewlett, Ph. D Chief, Laboratory of.

VIROLOGY. Viruses are the smallest infectious agents ( nm in diameter ) containing only one kind of nucleic acid (RNA or DNA) as their genome. The.

Trends in Biotechnology TB 14 Microinjection, stem cell transfer, gene targeting, and use of retroviruses 1.

Specific Cellular Defence.  Range of white blood cells (WBCs) circulate monitoring for damage, pathogens or cancerous cells  In response to damage or.

Antibodies I’ve heard of them but just what are they? Plasma Cells of Effector Cells Transcription Translation Polypeptide / Proteins Humoral Response:

MEDICAL TESTING Doctor requires information Patient sample collection

DIAGNOSIS OF HIV INFECTION THE LABORATORY BY DR. K.BUJJIBABU.MD.

Viruses, Viroids, and Prions

Perspectives on XMRV and Related Retroviruses John M. Coffin Endogenous proviruses Receptor usage Endogenous proviruses Receptor usage HIV Drug Resistance.

CA-125 Cancer Antigen-125 Discovered in l981

An Update on FDA/OBRR XMRV activities BPAC meeting, July 26, 2010 Indira Hewlett, Ph. D Chief, Laboratory of Molecular Virology DETTD/CBER/FDA.

Branches of Microbiology Bacteriology Virology Mycology Parasitology Immunology Recombinant DNA technology.

HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress JUAN MARTIN-SERRANO, TRINITY ZANG.

Viral Tissue Reservoirs Are Determined Early and Little Viral RNA Is Detected during Suppression in the Macaque Model Zandrea Ambrose, Ph.D. Division of.

APPLICATIONS OF MONOCLONAL ANTIBODIES

Viral Anatomy Envelope Forms from host membrane Forms from host membrane Viral genome includes genes for surface proteins Viral genome includes genes.

Xenotropic Murine Leukemia Virus-Related Retrovirus: CCR Assay Development.

Functional cure after long term HAART initiated during early HIV infection - a case study. van Lunzen J. 1,2, Schulze zur Wiesch J. 1,2, Schumacher U.

professor in microbiology

HUMAN IMMUNODEFICENCY VIRUS (HIV). INTRODUCTION HIV VIRUS IS HUMAN IMMUNODEFICENCY VIRUS IT CAUSES (ACQUIRED IMMUNODEFICENCY SYNDROME)(AIDS) HIV VIRUS.

Herpes and You By AJ Downs-Kingston & Julian Ward.

Immune Response and Immunity The Good Fight. Immune Response An immune response is when your body’s B-cells make antibodies against a particular antigen.

Immunity Feel free to review chapter 6.3 as well..

Retroviruses - Retroviridae

Chapter 2: The Path from HIV to AIDS

Volume 13, Issue 4, Pages (December 2006)

The human polysaccharide- and protein-specific immune response to Streptococcus pneumoniae is dependent on CD4+ T lymphocytes, CD14+ monocytes, and the.

Vlp construction The formation of virus-like particles (VLPs) is a result of self- assembly of one or several types of viral structural proteins, such.

Vlp construction The formation of virus-like particles (VLPs) is a result of self-assembly of one or several types of viral structural proteins, such as.

Poxviruses Poxviridae

Volume 53, Issue 5, Pages (May 1998)

KEY CONCEPT Genetics provides a basis for new medical treatments.

Chronic neutropenia mediated by Fas ligand

Volume 13, Issue 4, Pages (December 2006)

Volume 3, Issue 5, Pages (May 2008)

Volume 13, Issue 4, Pages (December 2006)

KEY CONCEPT Genetics provides a basis for new medical treatments.

KEY CONCEPT Genetics provides a basis for new medical treatments.

CD44-mediated neutrophil apoptosis in the rat

Volume 24, Issue 8, Pages (August 2018)

Volume 3, Issue 5, Pages (May 2008)

Histone Deacetylase Inhibitors Induce Apoptosis with Minimal Viral Reactivation in Cells Infected with Kaposi's Sarcoma-Associated Herpesvirus Andrea.

Humoral and Cell Mediated Immunity

Nef increases infectivity of HIV via lipid rafts

KEY CONCEPT Genetics provides a basis for new medical treatments.

KEY CONCEPT Genetics provides a basis for new medical treatments.

The human polysaccharide- and protein-specific immune response to Streptococcus pneumoniae is dependent on CD4+ T lymphocytes, CD14+ monocytes, and the.

Volume 13, Issue 5, Pages (November 2000)

Flow cytometric analysis of T cell activation.

Cell-surface expression of CD4 reduces HIV-1 infectivity by blocking Env incorporation in a Nef- and Vpu-inhibitable manner Juan Lama, Aram Mangasarian,

KEY CONCEPT Genetics provides a basis for new medical treatments.

Expression of MtrE by wild-type and mtr120 mutant strains.

Presentation transcript:

See using a flow cytometry assay expression of 3 goat poly clonal antibodies to purified viral proteins in both T and B cells but not in T cells from a normal donor.. We have shown expression of XMRV proteins but next wanted to ask if that reflected the presence of infectious and transmissable virus 3 goat polyclonal antibodies to purified viral proteins Rat α- MuLV p30 Gag mAb XMRV Protein Expression in Purified Activated T and B Lymphocytes from CFS Patients

Prostate cancer cell line Transmission of XMRV from Activated PBMCs of CFS Patients to the Human Prostate Cancer Cell Line LNCaP

Using a technique known as spinoculation we transmitted virus from plasma and confirmed infectious particles by TEM next slide 21 positive of 25 (84%) Cell Free Transmission of XMRV from CFS Patients Plasma to LNCap

Early and late buds type C morphology Mature extracellular viral particles with condensed centrally located nucleoid surrounded by an outer membrane separated by an electron lucent area Having detected the presence of XMRV viral proteins and particles in the PBMC of CFS patients, we next asked if we could detect an immune response to XMRV in CFS patients Infectious whole virus budding from the cell membrane Transmission Electron Micrograph of C-type Retrovirus Particles Transmitted from CFS patient T-Cells to LNCaP

BaF3-ER-SFFV Env BaF3-ER SFFV Env WB: αSFFV Env mAb BaF3-ERBaF3-ER-SFFV Env BaF3-ER BaF3-ER-SFFV Env Antibodies in CFS Patients Plasma to XMRV Env