Histology and Gross Anatomy of the Nervous System Lab 9 Histology and Gross Anatomy of the Nervous System
Lab 9 Activities Identify neuron, pyramidal cell, Purkinje cell, and peripheral nerve slides Identify boldfaced structures on brain models Identify specified parts in lab manual on sheep brain dissection Identify cranial nerves (by name + number I - XII)
Parts of a Neuron Soma Dendrites Axon
Cell Body (Soma) Contains the usual cellular organelles Primary site of synthesis & metabolism Cell membrane contains receptors to respond to stimuli Cell membrane transmits graded potentials Most cell bodies are located within the CNS clusters of neuron cell bodies in the CNS are called nuclei clusters of neuron cell bodies in the PNS are called ganglia
Dendrites Short, tapering, highly branched processes Not myelinated Contain some cell organelles Cell membrane contains receptors to respond to stimuli Transmit graded potentials towards the soma
Axons (nerve fiber) Originate at a cone-shaped region – the axon hillock A single process that transmits action potentials away from the soma May be long (1 meter) or short (1 mm) May branch (=collaterals) Collaterals end at axonal terminals synapse with neurons or effector cells (muscles or glands)
The Myelin Sheath Lipid-rich, covering of axons Oligodendrocytes in CNS Neurilemmocytes in PNS Dendrites are never myelinated Protects & electrically insulates Increases speed of nerve impulses 10-150x faster than unmyelinated fibers
structural Classifications Neurons structural Classifications
Multipolar Neuron (slide #24) Many processes originate from soma
Bipolar Neuron Two processes originate from soma
Unipolar Neuron A single process originates from soma
Neurons Specific types
Pyramidal Cells (slide #29)
Pyramidal Cells
Pyramidal Cells
Purkinje Cells (slide #30)
Purkinje Cells of Cerebellum
Purkinje Cells
Purkinje Cells cytoskeleton stain
Peripheral Nerve Connective tissue elements: Epineurium (wraps nerve) Perineurium (wraps fascicle) Endoneurium (wraps axon & myelin sheath)
Nerve, cross-section (slide #28) Epineurium Perineurium Endoneurium
Nerve, cross section, Fascicles Epineurium Perineurium Endoneurium
Nerve, cross section, Myelinated Fibers
Myelinated Axons, cross-section
Myelinated Axons, cross-section
Nerve, Long Section, 40X (slide # 28)
Nerve, long section, 100X
Neurofibrilar Node Nerve, 400X
Neurofibrilar Node, 1000X
Sheep Brain Dissection Dura mater partially removed
Sheep Brain Dissection and pia mater
Sheep Brain Dissection
Sheep Brain Dissection Note: pituitary removed
Sheep Brain Dissection
Sheep Brain in Cross Section
Key to Sheep Brain in Cross Section 1. Gray Matter 2. White Matter 3. Corpus Callosum 4. Lateral Ventricle 5. Caudate Nucleus 6. Septum Pellucidum 7. Fornix 8. Optic Chiasm 9. Third Ventricle 10. Thalamus 11. Corona Radiata 12. Hippocampus 13. Cerebral Aqueduct 14. Pituitary Gland 15. Pineal Gland
Human Cranial Nerves
Human Cranial Nerves
End of Lab 9 Presentation