Extraction of Nicotine By: DJ Hall and Brittany Hicks
Purpose Determine if nicotine is extractable by means of supercritical fluid Run more than one sample type to determine if this makes a significant difference
SFE Supercritical Fluid Extraction Uses high pressure CO 2 Used to extract organics from different types of matrixes Allows for easy separation Problems All soluble organics will be separated form matrix
GC-MS Gas Chromatography Mass Spectroscopy Separation of violate organics Determine the analyte separated at specific retention times using MS
Nicotine (162.12g/mol) Poison (parasympathomimetic alkaloid) Formed in nightshade family of plants Antiherbivor chemical Small doses Stimulant Relaxation Large doses Fatal
Extraction 2 Cigarettes Marlboro blend 27 2 Pieces Nicorette Gum 2mg 50 Drops of e-cigarette liquid 2.4% by weight
Extraction cont. All extractions were obtained by bubbling into methanol and using SFE methanol modifier pump Cigarettes Ground using mortar and pestle Nicorette Gum Cut into small pieces E-cigarette liquid Celite as inert matrix
Determination of Nicotine All samples were diluted to 50ml of methanol A standard was created by diluting 4mg of Nicotine in 50ml of methanol Standards and Samples were run using the GC-MS
Reference Mass Spectra
Standard Nicotine peak
Cigarettes Nicotine peak
Cigarettes cont.
E-Cigarette Liquid Nicotine peak
E-Cigarette Liquid Cont.
Nicorette Gum
Chromatogram Comparison E-Cigarette Liquid Nicorette Gum Cigarette Standard
Conclusion It is possible to extract nicotine using the SFE Both the E-Cigarette and Cigarette samples contained nicotine The Nicorette Gum showed undetectable amounts of nicotine Nicotine may have be contained in the gum residue that was filtered before dilution
Future Project Quantify the amounts of nicotine extracted Determine the extraction efficiency
Work Cited Fischer, M. and Jefferies, T. Optimization of Nicotine Extraction from Tobacco Using Supercritical Fluid Technology with Dynamic Extraction Modeling. J.Agric. Food Chem. (1996), 44,