AP Lab 4A Paper Chromatography

- technique that separates molecules from each other

Nonpolar pigments are carried along at different rates, b/c they are not equally soluble in the solvent. Nonpolar pigments are also attracted to different degrees to the fibers of the chromatography paper solvent nonpolar paper polar

AP Lab 4A Paper Chromatography Beta carotene = no polar groups (most non polar) Xanthophyl = 2 polar groups Chlorophyll a = 5 polar groups Chlorophyll b = 6 polar groups (most polar) CHROMOTOGRAPHY SOLVENT is NONPOLAR

AP Lab 4A Paper Chromatography polar pigments dissolve in polar pigments. LIKE DISSOLVES LIKE nonpolar pigments dissolve in nonpolar pigments.

AP Lab 4A Paper Chromatography polar pigments dissolve in polar pigments. CHROMOTOGRAPHY SOLVENT is NONPOLAR nonpolar pigments dissolve in nonpolar pigments.

AP Lab 4A Paper Chromatography Beta carotene = no polar groups (non polar) Xanthophyl = 2 polar groups Chlorophyll a = 5 polar groups Chlorophyll b = 6 polar groups (most polar) CHROMOTOGRAPHY PAPER is POLAR

AP Lab 4A Paper Chromatography As nonpolar solvent moves up the chromatography paper, pigments are dissolved to differing degrees and move along with it at differing degrees.

AP Lab 4A Paper Chromatography The more non-polar a pigment, the more soluble it is in a nonpolar solvent… The higher it will travel

Chromo pix here

Ratio Factor (this is a constant value)

Ratio Factor (this is a constant value) distance moved by pigment (mm)___ total distance moved by solvent (mm) =

(this is a constant value) distance moved by pigment (mm)_ total distance moved by solvent (mm) Ratio Factor =