Dr. Alvin Yeh Department of Biomedical Engineering Blood Harvest: Laboratory Creation of Blood Vessels Josh Bergerson Normangee High School

Virtual Model of Angiogenesis Important Process In: normal tissue repairmultiple diseasesengineered tissue.

In Vitro Angiogenesis Model Can study the effects of Varying collagen stiffness Varying collagen thickness Varying collagen concentration Endothelial cell monolayer Collagen gel preparation Serum–free medium

The Results

Electromagnetic spectrum Properties of waves Experimental design Optics & imaging PolymersFluorescenceMechanics PC data collection Elements of Research Applied in the Classroom

Relevance to Standards

Learning Objectives

Materials Borax Cardboard Silicon mold Computer paper Corn meal Sugar Salt Baking soda Clear glue Water Gelatin Fishing line Water Laser pointers(r/g/b) Meter stick Weight set Spring scale Ruler Beakers Craft sticks Binder clips Calculators Force sensor (opt) Light sensor (opt) Go-link(opt) scissors packing tape Food coloring (opt)

Classroom Lesson Day 1

Classroom Lesson Day 2 Matrix Constraints Must be smaller than 5cm x 5cm x 2cm Must contain fishing line Must be transparent Matrix Preparation Make mold (silicon/Cardboard) Mix polymer matrix Add additives (opt) Let gels set overnight  Corn meal  Salt  Sugar  Baking Powder

Classroom Lesson Day 3 Mechanical Testing Force sensor to measure x/y strength Mass set to test z strength Imaging the Sample Image with R/G/B Record diameter of sample Light sensor to gauge transparency of gel Testing the Matrix

Classroom Lesson Day 4 Image Analysis Use diffraction pattern to find diameter of sample Compare results of intensity with R/G/B Concluding Presentation Present matrix & data to class Suggest improvements they would make Compare class data to find most efficient model

Sample Pre- and Post- Test Questions Which of the following is the result of excessive angiogenesis? A stroke Cancer Tissue growth Tissue Repair Which color of visible light has the most energy? Red Green Blue Ultraviolet (True / False) Polymers with different chemical compositions may have different physical properties. (True / False) A polymer’s color is an example of an extensive property.

Sources From the Web uwbwritingcenter.wordpress.com en.wikipedia.org stacymakescents.com unschoolme.blogspot.com 0diameter%20of%20a%20hair%20using%20a%20laser.pdf 0diameter%20of%20a%20hair%20using%20a%20laser.pdf sensors/dfs-bta/ biology.clc.uc.edu

Acknowledgements TAMU E3 RET Program National Science Foundation Nuclear Power Institute Dr. Alvin Yeh Research Group: Tissue Microscopy Lab Dr. Arne Lekven Lab Group