Puccinia triticina virulence phenotypes present in Uruguay phenotypes present in Uruguay during 2004-2010 Silvia Germán La Estanzuela 2011 North American.

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Puccinia triticina virulence phenotypes present in Uruguay phenotypes present in Uruguay during Silvia Germán La Estanzuela 2011 North American Rust Workers Meeting St. Paul, Minnesta, USA June 12, 2011

Wheat leaf rust (Puccinia triticina)

Samples Trap nurseries Trap nurseries Experiments Experiments Crops Crops 1 – 2 single pustule isolates per sample 1 – 2 single pustule isolates per sample Differential set Lr 1 2a 2c 3 Lr Lr 3ka Lr Nomenclature Long and Kolmer, letter Prt code + Virulence to Lr10 and Lr20 MDT MDTMCR-10 MFP-20 MFP-20TDD-10,20 METHODOLOGY

Number of P. triticina isolates studied, tolal numer of races and races present with more than 5 and 10% frequency in Uruguay

Avirulence/virulence formulae of more frequent races, and year of first detection

Frequency of important Puccinia triticina isolates and affected cultivares Uruguay

Virulence formulae and year of first detection of other Puccinia triticina races present in Uruguay during during

% virulence % virulence Lr3bg high (2006 +) Lr14a 100 Lr14b high Lr21 0 Lr23 int-high Lr25 0 (2006 +, mixture) Lr39/ need confirm Lr need confirm Lr47 0 (2009 +) APR genes Lr12 inefective Lr13 ineffective Lr22a effective Lr22b ineffective Lr35 effective Lr37 ineffective Lr M Lr MSS Virulence frequency to Lr genes Field effective Lr29 0 Lr32 40 MRMS Lr33 60 MRMS Lr36 0 Lr45 0 Lr47 0 Lr51 0

Foto grupo de trabajo RichardGarcía NoeliaPerez FernandoPereira Thank you Gracias