1.The trp repressor is made of: A)DNA B)RNA C)Protein D)None of the above

2. The trp operator is made of: A)DNA B)RNA C)Protein D)None of the above

3. The lac repressor can bind to: A)Tryptophan B)cAMP C)Allolactose D)The lac operator E)Both C and D

4. What would happen to the expression of the trp operon if E. coli had a frameshift mutation in the trpR gene such that no trp Repressor protein was made? A) The cell would be unable to synthesize tryptophan B) The cell would express the Trp operon all the time C) The cell would express the Trp operon only when glucose is at low concentrations D) The cell would no longer need the trp operon

5. What would happen to the expression of the trp operon if E. coli had a missense mutation in the trpR gene such that the trp Repressor protein was unable to bind tryptophan, but otherwise functioned like the wild type version? A) The cell would be unable to synthesize tryptophan B) The cell would express the Trp operon all the time C) The cell would express the Trp operon only when glucose is at low concentrations D) The cell would no longer need the trp operon

6. What would happen to the expression of the lac operon if E. coli had a mutant version of the Lac Repressor that was unable to bind allolactose, but otherwise functioned like the wild type version? A) The lac operon would never be expressed B) The lac operon would expressed in low levels under some conditions, but never at high levels C) The lac operon would always be expressed, but would vary between low and high expression depending on the level of cAMP D) The lac operon would always be expressed, but would vary between low and high expression depending on the level of allolactose E) The lac operon would be expressed at a high level at all times

7. What would happen to the expression of the lac operon if E. coli had a mutant version of the Lac Operator that was unable to bind the Lac Repressor? A) The lac operon would never be expressed B) The lac operon would expressed in low levels under some conditions, but never at high levels C) The lac operon would always be expressed, but would vary between low and high expression depending on the level of cAMP D) The lac operon would always be expressed, but would vary between low and high expression depending on the level of allolactose E) The lac operon would be expressed at a high level at all times

8. What would happen to the expression of the lac operon if E. coli had a mutant version of the Catabolite Activator Protein (CAP) that was unable to bind cyclic AMP, but otherwise functioned like the wild type version? A) The lac operon would never be expressed B) The lac operon would expressed in low levels under some conditions, but never at high levels C) The lac operon would always be expressed, but would vary between low and high expression depending on the level of cAMP D) The lac operon would always be expressed, but would vary between low and high expression depending on the level of allolactose E) The lac operon would be expressed at a high level at all times