Bacterial genetics.

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Presentation transcript:

Bacterial genetics

Gram-positive bacteria transform DNA using a transformasome complex. Figure 9.1 3

Gene Transfer by Conjugation Conjugation is the transfer of DNA from one bacterium to another, following cell-to-cell contact. - It is typically initiated by a special pilus protruding from the donor cell. Figure 9.2 4

Figure 9.3 7

Figure 9.4 8

An integrated F-factor can excise from the chromosome. - Aberrant excision results in an F′ factor or F′ plasmid, which carries chromosomal genes. Figure 9.5 9

Hfr leu+ thr+pro+lac+bio+Sms X F- leu- thr-pro-lac-bio-SmR What medium should we use to obtain 1. leu+ transconjugants 2. pro+ transconjugants 3. lac+ transconjugants

Hfr leu+ thr+pro+lac+bio+Sms X F- leu- thr-pro-lac-bio-SmR time 1. leu+ transconjugants 2. pro+ transconjugants 3. lac+ transconjugants What are the map positions of leu, pro, and lac? time No.of colonies on medium 1 2 3 5 10 8 15 28 6 20 48 16 25 68 26 11

Generalized Transduction Figure 9.7 17

Figure 9.9 18

Specialized Transduction Figure 9.8 20

Generalized Recombination Figure 9.14 21

Figure 9.15 22

Figure 9.30 23

Mobile Genetic Elements IS elements transfer by one of 2 mechanisms: - Replicative or nonreplicative transposition Figure 9.31 24