SOIL SAMPLES FROM NORTH VIETNAM MOUNTAIN AS A KEY TO CLUE OCDD PHENOMENA Andrey Shelepchikov, Efim Brodsky, Olga Vasina Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow,Russia; Russian-Vietnamese Tropical Center, Hanoi, Vietnam

Location – north Vietnam, Lao Cai province, Sa Pa district, national park Hoang Lien

Hot-spot top (2005)Hot-spot top (2010)Below hot spot Ref. top Rain water accumulator 2,3,7,8-D<0,1<0,2 <0,4<0,2<0,09<0,030,02 <0,02<0,01 <0,03 1,2,3,7,8-D<0,2<0,31<0,33,31<0,211,40,070,250,020,06<0,01<0,02<0,05 1,2,3,4,7,8-D1,10,843,314,20,474,20,17<0,040,030,04<0,01<0,03 1,2,3,6,7,8-D1,31,23,520,30,727,60,161,950,070,27<0,010,09<0,05 1,2,3,7,8,9-D6,01,26,321,91,1912,00,762,140,350,54<0,020,800,67 1,2,3,4,6,7,8-D27328, ,843152,71071,601,260,070,410,62 OCDD ,92,13,512,7 2,3,7,8-F<0,08<0,16<0,15<0,3<0,1<0,04<0,02 <0,07<0,03<0,01 <0,03 1,2,3,7,8-F<0,1<0,2<0,19<0,3<0,1<0,06<0,04<0,030,010,06<0,01 <0,03 2,3,4,7,8-F<0,1<0,2<0,19<0,3<0,1 <0,04<0,03<0,02<0,04<0,01<0,02<0,07 1,2,3,4,7,8-F<0,12,2<0,2<0,3<0,20,090,04 0,010,070,01 <0,02 1,2,3,6,7,8-F<0,11,40<0,2<0,3<0,20,130,03<0,030,020,090,010,02<0,05 1,2,3,7,8,9-F<0,11,60<0,2<0,4<0,20,12<0,02<0,030,010,27<0,01<0,02<0,04 2,3,4,6,7,8-F<0,20,64<0,3<0,4<0,20,05<0,04<0,030,010,040,01<0,02<0,05 1,2,3,4,6,7,8-F0,947,1<0,2<0,4<0,20,760,370,210,140,380,130,060,13 1,2,3,4,7,8,9-F<0,21,4<0,3<0,6<0,20,04<0,040,02<0,010,020,01<0,01<0,03 OCDF1,157,6<0,2<0,3<0,10,480,270,210,070,230,080,060,09 Total TCDDs2,73,212127,23,91,60,060,400,140,5<0,05<0,09<0,2 Total PeCDDs<0,2<0,317,7552,317,50,225,10,090,55<0,06<0,02<0,25 Total HxCDDs ,11613,938381,72,10,042,91,4 Total HpCDDs ,13,30,150,81,2 Total TCDFs4,92,72,052,12,90,680,030,25<0,11,6<0,07<0,1<0,3 Total PeCDFs<0.2<0,2<0.20,6<0.20,28<0,2<0,30,130,46<0,08<0,1<0,3 Total HxCDFs<0.214<0.20,6<0.22,10,27 0,190,820,09 <0,2 Total HpCDFs<0,34,3<0,30,6<0,31,10,450,250,200,510,170,060,17 PCP/PCP salt PCDD/Fs and PCP concentration in soil samples FactSupposition The OCDD hot-spot - flat top of a hill (~100 м 2, above sea level ~ 2120 m); most likely original pollution was dotted. The neighbor top (~500 m on a straight line) is not polluted Forest fire was not long ago. Only trace amount of PCDFs were found Neither chlorinated compounds has not been found in samples on OCDD concentration level PCP level in the hot-spot is low, but higher than in other soil samples OCDD could not be formed in technological processes without PCDFs Historical contamination or atmospheric deposition accumulation are less probable The source of pollution has been brought by the human Forest's fire could not be a course of contamination The fragments the plastic bag for loose goods have been found on the hot-spot PCP salt and it’s water soluble impurities could be eluted by rains. If we accept that OCDD pollution is caused by PCP, we should allow that stability of OCDD is much higher than other PCDD/Fs or OCDD could form in-situ in soil. Plastic bag could contain some chemicals. Hot-spot top (2005)Hot-spot top (2010)Below hot spot Ref. top Rain water accumulator 2,3,7,8-D<0,1<0,2 <0,4<0,2<0,09<0,030,02 <0,02<0,01 <0,03 1,2,3,7,8-D<0,2<0,31<0,33,31<0,211,40,070,250,020,06<0,01<0,02<0,05 1,2,3,4,7,8-D1,10,843,314,20,474,20,17<0,040,030,04<0,01<0,03 1,2,3,6,7,8-D1,31,23,520,30,727,60,161,950,070,27<0,010,09<0,05 1,2,3,7,8,9-D6,01,26,321,91,1912,00,762,140,350,54<0,020,800,67 1,2,3,4,6,7,8-D27328, ,843152,71071,601,260,070,410,62 OCDD ,92,13,512,7 2,3,7,8-F<0,08<0,16<0,15<0,3<0,1<0,04<0,02 <0,07<0,03<0,01 <0,03 1,2,3,7,8-F<0,1<0,2<0,19<0,3<0,1<0,06<0,04<0,030,010,06<0,01 <0,03 2,3,4,7,8-F<0,1<0,2<0,19<0,3<0,1 <0,04<0,03<0,02<0,04<0,01<0,02<0,07 1,2,3,4,7,8-F<0,12,2<0,2<0,3<0,20,090,04 0,010,070,01 <0,02 1,2,3,6,7,8-F<0,11,40<0,2<0,3<0,20,130,03<0,030,020,090,010,02<0,05 1,2,3,7,8,9-F<0,11,60<0,2<0,4<0,20,12<0,02<0,030,010,27<0,01<0,02<0,04 2,3,4,6,7,8-F<0,20,64<0,3<0,4<0,20,05<0,04<0,030,010,040,01<0,02<0,05 1,2,3,4,6,7,8-F0,947,1<0,2<0,4<0,20,760,370,210,140,380,130,060,13 1,2,3,4,7,8,9-F<0,21,4<0,3<0,6<0,20,04<0,040,02<0,010,020,01<0,01<0,03 OCDF1,157,6<0,2<0,3<0,10,480,270,210,070,230,080,060,09 Total TCDDs2,73,212127,23,91,60,060,400,140,5<0,05<0,09<0,2 Total PeCDDs<0,2<0,317,7552,317,50,225,10,090,55<0,06<0,02<0,25 Total HxCDDs ,11613,938381,72,10,042,91,4 Total HpCDDs ,13,30,150,81,2 Total TCDFs4,92,72,052,12,90,680,030,25<0,11,6<0,07<0,1<0,3 Total PeCDFs<0.2<0,2<0.20,6<0.20,28<0,2<0,30,130,46<0,08<0,1<0,3 Total HxCDFs<0.214<0.20,6<0.22,10,27 0,190,820,09 <0,2 Total HpCDFs<0,34,3<0,30,6<0,31,10,450,250,200,510,170,060,17 PCP/PCP salt

The samples were analyzed by HRGC/HRMS (НР 6890 Plus / Finnigan MAT 95XP with an upgraded cooling system) using SGE BPX-5 column. PCDD/Fs were analyzed by typical procedure. Full-scan experiments were run at different scan rate and m/z interval, in order to obtain the most detail mass-spectrum. Mass measuring precision was controlled by 13 C 6 -PCP, D 8 -DDD and native OCDD ions; in most full-scan runs, it was ± 0,02amu. We analyze organic (toluene:acetone) and basic extracts from soil samples without any clean-up or after passing through the micro column with sulfuric acid on silica gel. TIC chromatogram and OCDD mass-spectrum of soil sample from OCDD hot-spot (m/z , 2s/d, R=8000). unidentified

Identification criteria: Exact m/z Characteristic EI mass fragmentation pattern with presence at least five isotope ion clusters corresponding loss of neutral particles СН 3, (СН 3 +СО) and Cl Rupture of Ar-O-Ar bond at biphenyl ethers True isotopic ratio for 4-5 main ions in molecular and fragmental clusters

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