Optical Tweezers Lincoln Connor Maria Conneran. Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation.

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Presentation transcript:

Optical Tweezers Lincoln Connor Maria Conneran

Outline Project Goals Optical tweezing theory and results Sample preparation Quadrant photodetector motivation and results Future work

Goals CCD imaging of optical trapping Implement quadrant photodetector Reorient detection wing Manipulate biological sample

Setup

Optical Tweezing Theory Different indices of refraction Conservation of momentum Equilibrium R.Koebler optical trap/optical tweezer: explanation with unfocused laser

Applications for Optical Tweezers Hold, measure/apply known force, local viscosity; Parthasarathy Lab:McIntyre Lab:

Optical Trapping Beam expander Overfill back aperture Video

Sample Preparation Spacer Sealants Cleaning Dilution Sticky tape wells Higher viscosity immersion oil

Dilution 1/1600

Quadrant Photodetector Silicon ( nm) X, Y, SUM 150kHz bandwidth Responsivity: 0.45A/W Dark Current of 5nA Recommended spot size: 1 mm to 3.9 mm

Calibration 5.37μm/V Piezoelectric Voltage

Equipartition

Lorentzian Fit

Suggestions for future work Improve sample preparation Improve stability Detection beam SLM implementation

Questions?

Horizontal Setup

Lens System for QPD

Data Acquisition

QPD DAQ

GigE Camera Olympus 100x oil immersion objective lens

Einstein-Ornstein-Uhlenbeck

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