Color Test for Specific Amino Acids

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Presentation transcript:

Color Test for Specific Amino Acids Done by :Sahar Al-Subaie

Millon's Test Hopkins-Cole Test Sakaguchi Test Outlines: Millon's Test Hopkins-Cole Test Sakaguchi Test

Millon's Test

Used for detecting the presence of monohydroxybenzene derivatives Aim: Used for detecting the presence of monohydroxybenzene derivatives 

monohydroxybenzene derivatives: Tyrosine, Tyrosine derivatives, Phenol

Principle: Phenolic group of tyrosine +Millon's reagent red color (mercury salt of nitrated tyrosine)

Millon's reagent: It is a solution of mercuric and mercurous ions in nitric and nitrous acids.

Hopkins-Cole Test

This test aims to detect Tryptophan (indole ring)

Tryptophan + Hopkins-Cole H2SO4 purple ring Principle: Tryptophan + Hopkins-Cole   H2SO4 purple ring

Sakaguchi Test

This test is specific for the arginine (guanidine group) Aim: This test is specific for the arginine (guanidine group)

arginine +α-Naphthol NaOBr red color Principle: arginine +α-Naphthol NaOBr red color

Thank you

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