Prepared by Lawrence Kok Video Tutorial on Chromatography, Paper, Thin Layer, Column Chromatography, HPLC and GLC.

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Presentation transcript:

Prepared by Lawrence Kok Video Tutorial on Chromatography, Paper, Thin Layer, Column Chromatography, HPLC and GLC.

Click here to view TLC and Column Chromatography Separation using TLCTLC techniques step by step Column ChromatographyColumn chromatography separation

Click here to view HPLC and Gas Liquid Chromatography HPLC worksGas Liquid Chromatography works

Chromatography Partition Chromatography vs Adsorption Chromatography

Partition Chromatography Adsorption Chromatography Pic taken from: 1

Pic taken from: Mobile phase - Liquid Stationary phase - LiquidStationary phase - Solid

Partition Chromatography Adsorption Chromatography 2 Distribution of X, Y and Z between two liquid phases Adsorption of X, Y and Z onto SOLID phase

Type of chromatography

Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase) Stationary phase – Solid silica SiO 2 or alumina AI 2 O 3 (solid phase) Stationary phase – Solid silica SiO 2 or alumina AI 2 O 3 packed in column (solid phase)

Type of chromatography Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase) Stationary phase – Solid silica SiO 2 or alumina AI 2 O 3 (solid phase) Stationary phase – Solid silica SiO 2 or alumina AI 2 O 3 packed in column (solid phase) Mobile phase is liquid solvent

Type of chromatography Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase) Stationary phase – Solid silica SiO 2 or alumina AI 2 O 3 (solid phase) Stationary phase – Solid silica SiO 2 or alumina AI 2 O 3 packed in column (solid phase) Mobile phase is liquid solvent Solvent moves by capillary action Partition/Distribution of solute between 2 liquid phase Adsorption of solute onto Stationary solid phase Adsorption of solute on Stationary phase - strongly adsorp – travel slowly (eluted last) - weakly adsorp – travel fast (eluted first)

Separated components measured using Rf values 1 2

Click here to view Separation using TLCTLC techniques step by step Column ChromatographyColumn chromatography separation

Type of chromatography

We also have HPLC and GLC

Pic from: / / Pic from: High Performance Liquid Chromatography Gas Liquid Chromatography

Click here to view HPLC worksGas Liquid Chromatography works

Summary Paper Chromatography - Partition Chromatography - Mobile phase (Liquid) - Stationary phase (Liquid) Thin Layer Chromatography - Adsorption Chromatography - Mobile phase (Liquid) - Stationary phase (Solid) Column Chromatography - Adsorption Chromatography - Mobile phase (Liquid) - Stationary phase (Solid) High Performance Liquid Chromatography - Partition Chromatography - Mobile phase (Liquid) - Stationary phase (Liquid) Gas Liquid Chromatography – Partition Chromatography - Mobile phase (Gas) - Stationary phase (Liquid)

Acknowledgements Thanks to source of pictures and video used in this presentation Thanks to Creative Commons for excellent contribution on licenses Prepared by Lawrence Kok Check out more video tutorials from my site and hope you enjoy this tutorial

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