Histology Muscle Lab Stephen Ernst, Ph.D. / Matt Velkey, Ph.D.

58 Skeletal Muscle Longitudinal Striated Muscle Fiber (cell) Endomysium Peripheral Flattened Nuclei Striations

2 consecutive Z line = Sarcomere 58L Skeletal Muscle Dark A Band (myosin) Light I Bands (actin) Divided by Z Line 2 consecutive Z line = Sarcomere

Slide 58 Skeletal Muscle Cross Section Peripheral Nuclei Muscle Fibers

Slide 59 H&E Skeletal Muscle Peripheral Nerve (Use this slide to examine CT layers in skeletal muscle; ignore, nerve fasicles which are also present) Skeletal Muscle Peripheral Nerve

Slide 59 Skeletal Muscle Cross Section H&E Stain Individual Muscles Tendon Epimysium Perimysium SKIN: Epimysium Epidermis Adipose Tissue Dermis

Slide 59 Trichrome Stain Endomysium Perimysium

Slide 71 (even) Axons Muscle fibers (purple) Neuromuscular junctions

Slide 71 (even) Neuromuscular Junctions (a.k.a. motor end plates)

Slide 71 (even) Axon Node of Ranvier Neuromuscular junction

Slide 71 (even) Axon Neuromuscular junction (viewed from top)

Slide 71 (even) Axon Neuromuscular junction (viewed from top)

Skeletal muscle Neuromuscular spindle Slide 71 (odd)

Slide 71 (odd) outer capsule of neuromuscular spindle inner capsule Intrafusal fibers Skeletal muscle (extrafusal fibers)

NOT a neuromusclar spindle! Slide 71 (odd) Skeletal muscle (extrafusal fibers) Peripheral nerve NOT a neuromusclar spindle!

57 Cardiac Muscle Longitudinal Fibers Cross Sectioned Fibers (Note: Slide 98 is also quite good for Intercalated disks) Longitudinal Fibers Cross Sectioned Fibers branching Intercalated Disk Large Nuclei centrally placed

29 Smooth Muscle (Intestine) Cross Section Loose Connective Tissue Small,Fusiform (spindle shaped)Fiber Central Flattened Nucleus Longitudinal Section

Cross Section Smooth Muscle Fasicle 250 Vagina Smooth Muscle Cross Section Smooth Muscle Fasicle CT CT Longitudinal Smooth Muscle Fasicle CT

250 High Power Smooth Muscle Fasciles CT