Genetics of Organisms AP Lab 7.

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Presentation transcript:

Genetics of Organisms AP Lab 7

Far Side, Gary Larson…

Background Drosophila melanogaster is a model organism used often in genetics experiments. Why? Short reproductive cycle Produces large numbers of offspring Small, easy to maintain Easy to observe traits

Images of Drosophila http://www.mun.ca/biology/scarr/Drosophila_eyes.gif http://flybase.bio.indiana.edu/.bin/imagereport.html?FBim0000001

Mock lab Since performing experiments with real flies will take too long, we will use mock data to complete the lab. Assume our research was completed on the wild type- (red) versus white-eyed flies. This is an example of sex-linked inheritance

The code We will use the following shorthand to represent eye color alleles: XR Wild-type X Xr White X Y

White eyed is recessive Some facts… Female = XX Male = XY Wild-type is dominant White eyed is recessive http://www.accessexcellence.org/RC/VL/GG/sex.html

Mock data P generation = Xr Xr x XRY Table 7.1 F1 Generation Data Phenotype and symbol Females Males Red eye All XR Xr White eye All XrY

Mock data, part 2 F1 cross: XR Xr x XrY Phenotype and symbol Females Red eye 78 XR Xr 69 XRY White eye 62 Xr Xr 73 XrY

Analysis of Results Answer questions 1-6 in your lab packet. Remember… Expected ratios are what you predict will happen based on probability and Punnett Squares Observed ratios are what you actually get from mating the flies

Analysis of Results, part 2 Question 7: fill in the first 5 columns, and read ahead about Chi-Square. Then answer the three discussion questions. Homework: Preview the Statistical Analysis section and fill in as much as you can. Write down any questions you have. This is where we will pick up next time.