HPLC Systems. Column Chromatography HPLC Modes HPLC – System Components.

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Presentation transcript:

HPLC Systems

Column Chromatography

HPLC Modes

HPLC – System Components

HPLC – Pump Head Piston HPLC Pump Head

Gradient HPLC High Pressure Low Pressure

Chromatography – Dead Volume Mixing

HPLC Ion Separation Mode

HPLC Solvents Mobile Phase

HPLC – Solvents Properties

HPLC – Solvents Groups

HPLC – Solvents Polarity for Reverse Phase

HPLC – Solvent Effect

HPLC – Effect of Modifier 60:40 50:50 40:60 Acetonitrile : Water

HPLC – Gradient Curves

HPLC Columns

Mechanism of separation in different forms of HPLC

HPLC – Effect of Particles Size

HPLC – Particles Size

HPLC – Porous and Solid Packing

HPLC – Effect of Carbon Chain Length on Separation

HPLC – Column Diameter and Separation

Size Exclusion HPLC

HPLC Detectors

HPLC – Diode Array Detector

HPLC – UV/VIS Spectrophotometer

HPLC – Diode Array Detector Output

HPLC – UV/VIS Detector

HPLC – Spectrometric Detector Flow Cell Design

HPLC – UV Detector Cell

HPLC – Fluorescence Detector

HPLC - ELSD

HPLC - Evaporative Light Scattering Detector

HPLC - Detectors

HPLC – Derivatization for Detection

HPLC - Applications