MICHAEL HOOKER MICROSCOPY Core Facility—open to all UNC investigators Confocal – fluorescence/reflection/frap/fret Atomic Force Microscope Laser Micro-Dissection Light Microscopy – tans./fluor./DIC/phase/multimode/time-lapse Mike Chua

MICROSCOPY SERVICES UNC Core facility Confocal Laser Capture Microscopy Electron Microscopy Light Microscopy Bob Bagnell

FLOW CYTOMETRY CORE UNC Core Facility Deconvolution System (3D) Laser Scanning Cytometer FACS Larry Arnold

R. SUPERFINE - T. SALMON LABS Scanning Electron Microscopy Transmitted Electron Microscopy Atomic Force Microscopy 3D Force Microscopy Confocal Microscopy Light Microscopy

RUSSELL TAYLOR- VISUALIZATION LAB Scientific Visualization Distributed Virtual Worlds Haptic Display Interactive 3D Computer Graphics UNC NIH National Research Resources for Computer Integrated Systems for Microscopy and Manipulation-- mm/

UNC NEUROIMAGING LABORATORY G. Gerig, A. Belger, J. Lieberman, J. Piven Cognitive Neuroscience –ERP lab: Functional brain mapping –Functional brain mapping using fMRI Analysis of MRI diffusion tensor imaging (DTI) 3D ultrasound: Quantitative image analysis Developmental Neuroimaging: UNC-NDRC Core High-power computer system dedicated to neuroimage analysis Neurolab: NIRL: NDRC:

BARRY WHITSEL LAB Intrinsic Optical Signal Imager (cell-based, in vivo)

BIOLOGY Kerry Bloom – Ted Salmon – Tony Perdue Spinning disk Confocals Laser Scanning Confocals Transmission Electron Microscopy

BIOMEDICAL ENGINEERING MRS Metabolomics Imaging (J. MacDonald) Cortical Intrinsic Signal Imaging Multiphoton fluorescence Imaging Transmembrane Potential Imaging Intracellular Calcium Imaging Biophotonics Computer Imaging

CELL & DEVELOPMENTAL BIOLOGY J. LeMasters – J. Costello – K. Jacobson Confocal – Single & 2 Photon Cryo – Transmitted Electron Microscopy Scanning Electron Microscopy Optical Tweezers Fluorescence Recovery After Photobleaching

CHEMISTRY Nuclear Magnetic Resonance Spectroscopy Total Internal Reflection Microscopy – Nancy Thompson

DENTAL SCHOOL Transmitted Electron Microscopy Scanning Electron Microscopy Wallace Ambrose

PEDIATRICS/EPA—J. CARSON Freeze Fracture Transmitted Electron Microscopy Scanning Electron Microscopy Light Microscopy

RADIATION ONCOLOGY Edward Chaney CT Segmentation Registration 3D & 4D Display

RADIOLOGY – RESEARCH Weili Lin– Etta Pisano – Stephen Aylward 3 Tesla Magnetic Resonance Imaging (Lin) Micro Single-Photon Computed Tomography (Lin) Diffraction Enhanced Imaging(Pisano) Computer Aided Diagnosis and Display Lab (CADDLab: Aylward) -- 2D and 3D image segmentation and registration --Multi-modal image resgistration

COMPUTER-ASSISTED SURGERY & IMAGING LAB (CASILAB) Elizabeth Bullitt Vessel/Tumor Imaging and Analysis Imaged Guided Surgery

GENE THERAPY CENTER Optical Imaging (Cryocooled, luminescence and fluorescence) Allison Hawke

LINEBERGER COMPREHENSIVE CANCER CENTER Light Microscopy Fluorescence Microscopy Steve Oglesbee

NEUROSCIENCES & NEURODEVELOPMENTAL DISORDERS RESEARCH CENTERS Eva Anton - Robert Sealock Confocals – Single & 2-Photon Light Microscopy

VASCULAR BIOLOGY CENTER Cam Patterson Ultrasound for small animals

MEDICAL IMAGE DISPLAY & ANALYSIS GROUP (MIDAG) At Comp Sci, Psychiatry, Rad Onc, Radiology, Surgery Analysis Capabilities Image Analysis Image Display Augmented Reality (Computer Science) Segmentation Registration

NC STATE UNIVERSITY BOTANY DEPARTMENT – Light Microscopy, Confocal, Nina Allen, Box 7612, NCSU John MacKenzie – Coordinator, EM Center Phillip Russell – Dir, Analytical Instru. Facility Atomic Force & TEM, Michael Dykstra – Dir, Laboratory Advanced Microscopy, TEM & Confocal VET SCHOOL – X-ray, CT, ultrasound, MRI Ian Robertson,

DUKE-UNC BRAIN IMAGING & ANALYSIS CENTER (BIAC) fMRI Neuroscience acquisition and analyses

POSSIBLE APPLICATIONS (from NIH proposal – T. Van Dyke & B. Tsui) High-resolution pinhole SPECT studies: Can do a series of images with a single injection depending upon the length of the half-life of the radionucleotide. Locating tumors and bone metastases microPET studies: Locating a large variety of primary tumors and their metastases

POSSIBLE APPLICATIONS (from NIH proposal – T. Van Dyke & B. Tsui) MRI and MRS studies: Tumor growth Changes in tumor vascularity Optical Imaging studies: Luciferase localization Reporter gene expression MicroCT studies: Tumor growth

FUTURE DEVELOPMENTS (from NIH proposal – T. Van Dyke & B. Tsui) Use Animal Model systems for refining specific imaging technologies—i.e. technologies to measure blood flow and microvascular permeability within tumors. Imaging results compared to histologic analyses can help develop technologies to measure disease progression and then be used to screen development of disease in backgrounds with additional genetic alterations.