Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-1The biosynthetic origins of purine ring atoms. Page 1069.

Slides:

Advertisements

Similar presentations

FCH 532 Lecture 29 Chapter 28: Nucleotide metabolism

Advertisements

Nucleotide Metabolism C483 Spring A ribose sugar is added to ________ rings after their synthesis and to ________ rings during their synthesis.

OBJECTIVES: 1.Nomenclature of nucleic acids: a. nucleosides* b. nucleotides 2.Structure and function of purines and pyrimidines. 3.Origin of atoms in.

Nucleotide Metabolism Student Edition 6/3/13 version Pharm. 304 Biochemistry Fall 2014 Dr. Brad Chazotte 213 Maddox Hall Web Site:

Nucleotide Metabolism.

Nucleic Acids Metabolism

Principles of Biochemistry

Metabolism of purines and pyrimidines - exercise - Vladimíra Kvasnicová.

BIOC Dr. Tischler Lecture 20 – February 10, 2006 METABOLISM: NUCLEOTIDE SYNTHESIS & DISORDERS.

1 Nucleotide Metabolism Nisa Rachmania Mubarik Major Microbiology Department of Biology, IPB 1212 Microbial Physiology (Nisa RM) ATP, are the sources of.

Nucleic Acid Metabolism Robert F. Waters, PhD

Chapter 26 Synthesis and Degradation of Nucleotides

Chapter 27 The Synthesis and Degradation of Nucleotides to accompany

Nucleic acids metabolism

Final Exam Mon 8 am.

February 19 Chapter 27 Nucleic acid metabolism

Anabolism of Nitrogen Compounds

Figure The citric acid cycle in context.

Unless otherwise noted, the content of this course material is licensed under a Creative Commons Attribution – Share Alike 3.0 License. Copyright 2007,

PRPP synthetase Nucleoside phosphorylases Phosphoribosyl transferases.

Chapter 26 The Synthesis and Degradation of Nucleotides Biochemistry

Metabolism of amino acids, purine and pyrimidine bases

UNIT IV: Nitrogen Metabolism Nucleotide Metabolism Part 2.

Purine Degradation & Gout (Musculoskeletal Block)

17. Amino acids are precursors of many specialized biomolecules

Biosynthesis of nucleotides Natalia Tretyakova, Ph.D. Phar 6152 Spring 2004 Required reading: Stryer’s Biochemistry 5 th edition, p , (or.

Nucleotide Metabolism

Nitrogen Fixation Nitrogen fixation is the reduction of ____________:

Lecture 30 Pyrimidine Metabolism/Disease Raymond B. Birge, PhD.

Nucleotide metabolism

Synthesis and Degradation of Nucleotides Part 2: September 2 nd, 2009 Champion CS Deivanayagam Center for Biophysical Sciences and Engineering University.

FCH 532 Lecture 31 Chapter 28: Nucleotide metabolism

FCH 532 Lecture 27 Chapter 28: Nucleotide metabolism Quiz on Monday (4/18) - IMP biosynthesis pathway ACS exam has been moved to Monday (5/2) Final is.

Metabolism of purine nucleotides A- De Novo synthesis: of AMP and GMP Sources of the atoms in purine ring: N1: derived from NH2 group of aspartate C2 and.

Nucleotide Metabolism

Nucleotide Metabolism -Biosynthesis-

BIOC/DENT/PHCY 230 LECTURE 6. Nucleotides o found in DNA and RNA o used for energy (ATP and GTP) o building blocks for coenzymes (NADH)

Nucleotide Metabolism -Biosynthesis- Dr. Sooad Al-Daihan 1.

FCH 532 Lecture 29 Chapter 28: Nucleotide metabolism Chapter 24: Photosynthesis New study guide posted.

: Nitrogen metabolism Part B Nucleotide metabolism.

PURINE & PYRIMIDINE METABOLISM dr Agus Budiman. Nucleotide consists purine / pyrimidine base, ribose/deoxyribose and phosphates. Nucleotide consists purine.

FCH 532 Lecture 28 Chapter 28: Nucleotide metabolism

Central Dogma of Biology. Nucleic Acids Are Essential For Information Transfer in Cells  Information encoded in a DNA molecule is transcribed via synthesis.

Metabolism of purines and pyrimidines Vladimíra Kvasnicová The figure was found at (Jan 2008)

Nucleotide metabolism

Nucleotide Metabolism

Chapter 8. Nucleotide Metabolism

Metabolism of purines and pyrimidines

Coenzymes and cofactors A large number of enzymes require an additional non ‑ protein component to carry out its catalytic functions called as cofactors.

Metabolism of purine nucleotides A- De Novo synthesis: of AMP and GMP Sources of the atoms in purine ring: N1: derived from NH2 group of aspartate C2 and.

Purine – Lecture. Nucleotides play key roles in many, many cellular processes 1. Activated precursors of RNA and DNA 2. Adenine nucleotides are components.

Nucleotides & Nucleic Acids RNA structure Single-stranded (ss) RNAs.

5’-IMP 5’-XMP 5’-GMP GDP GTP dGDP dGTP 5’-AMP ADP ATP dADP dATP.

Functions of Nucleotide: 1.Responsible for transmission of genetic informations 2. Act as energy currency 3.Carrier molecule for a broad spectrum of functional.

Chapter Twenty-Three The Metabolism of Nitrogen. Nitrogen Fixation Nitrogen fixation is the reduction of N 2 to NH 3: Bacteria are responsible for the.

Chapter Twenty-Three The Metabolism of Nitrogen. Nitrogen Fixation Nitrogen fixation is the reduction of N 2 to NH 3 : Bacteria are responsible for the.

Metabolism of purine nucleotides A- De Novo synthesis: of AMP and GMP Sources of the atoms in purine ring: N1: derived from NH2 group of aspartate C2 and.

Nucleotide Metabolism

Synthesis of Pyrimidine Nucleotides

Conversion of IMP to AMP

Nucleotide Metabolism

Nucleotides: Synthesis and Degradation

Pyrimidine metabolism

Pyrimidine Synthesis and Degradation

Pu & Py salvage Title slide..

Deoxyribonucleotide production

Synthesis of Pyrimidine Nucleotides

Figure 20.1 Synthesis of PRPP.

ATP? Depends on the system…..

Chapter 8. Nucleotide Metabolism

Presentation transcript:

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-1The biosynthetic origins of purine ring atoms. Page 1069

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-2The metabolic pathway for the de novo biosynthesis of IMP. Page 1071

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-3The proposed mechanism of formylglycinamide ribotide (FGAM) synthetase. Page 1072

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-4IMP is converted to AMP or GMP in separate two-reaction pathways. Page 1074

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-5Control network for the purine biosynthesis pathway. Page 1075

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-6The biosynthetic origins of pyrimidine ring atoms. Page 1077

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-7Metabolic pathway for the de novo synthesis of UMP. Page 1077

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-8Reactions catalyzed by eukaryotic dihydroorotate dehydrogenase. Page 1078

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-9Proposed catalytic mechanism for OMP decarboxylase. Page 1079

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-10Synthesis of CTP from UTP. Page 1080

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-11Regulation of pyrimidine biosynthesis. The control networks are shown for (a) E. coli and (b) animals. Page 1080

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-12aClass I ribonucleotide reductase from E. coli. (a) A schematic diagram of its quaternary structure. Page 1082

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-12bClass I ribonucleotide reductase from E. coli. (b) The X-ray structure of R2 2. Page 1082

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-12cClass I ribonucleotide reductase from E. coli. (c) The binuclear Fe(III) complex of R2. Page 1082

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-12dClass I ribonucleotide reductase from E. coli. (d) The X-ray structure of the R1 dimer. Page 1082

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-13Enzymatic mechanism of ribonucleotide reductase. Page 1083

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-14aRibonucleotide reductase regulation. (a) A model for the allosteric regulation of Class I RNR via its oligomerization. Page 1085

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-14bRibonucleotide reductase regulation. (b) The X-ray structure of the R1 hexamer, which has D 3 symmetry, in complex with ADPNP as viewed along its 3-fold axis. Page 1085

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-14cRibonucleotide reductase regulation. (c) The R1·ADPNP hexamer as viewed along the vertical 2-fold axis in Part b. Page 1085

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-15X-Ray structure of human thioredoxin in its reduced (sulfhydryl) state. Page 1086

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-16Electron-transfer pathway for nucleoside diphosphate (NDP) reduction. Page 1087

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-17aX-Ray structures of E. coli thioredoxin reductase (TrxR). (a) The C138S mutant TrxR in complex with NADP +. Page 1087

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-17bThe C135S mutant thioredoxin reductase (TrxR) in complex with AADP +, disulfide-linked to the C35S mutant of Trx. Page 1087

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-18aX-Ray structure of human dUTPase. (a) The molecular surface at the substrate binding site showing how the enzyme differentiates uracil from thymine. Page 1089

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-18bX-Ray structure of human dUTPase. (b) The substrate binding site indicating how the enzyme differentiates uracil from cytosine and 2-deoxyribose from ribose. Page 1089

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-19Catalytic mechanism of thymidylate synthase. Page 1090

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-20The X-ray structure of the E. coli thymidylate synthase–FdUMP–THF ternary complex. Page 1091

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-21Regeneration of N5,N10- methylenetetrahydrofolate. Page 1091

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-22Ribbon diagram of human dihydrofolate reductase in complex with folate. Page 1091

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-23Major pathways of purine catabolism in animals. Page 1093

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-24aStructure and mechanism of adenosine deaminase. (a) A ribbon diagram of murine adenosine deaminase in complex with its transition state analog HDPR. Page 1094

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-24bStructure and mechanism of adenosine deaminase. (b) The proposed catalytic mechanism of adenosine deaminase. Page 1094

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-25The purine nucleotide cycle. Page 1095

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-26aX-Ray structure of xanthine oxidase from cow’s milk in complex with salicylic acid. (a) Ribbon diagram of its 1332-residue subunit. Page 1095

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-26bX-Ray structure of xanthine oxidase from cow’s milk in complex with salicylic acid. (b) The enzyme’s redox cofactors and salicylic acid (Sal). Page 1095

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-27Mechanism of xanthine oxidase. Page 1096

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-28Degradation of uric acid to ammonia. Page 1097

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-29The Gout, a cartoon by James Gilroy (1799). Page 1097

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-30Major pathways of pyrimidine catabolism in animals. Page 1098

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-31Pathways for the biosynthesis of NAD + and NADP +. Page 1099

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-32Biosynthesis of FMN and FAD from the vitamin precursor riboflavin. Page 1100

Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 28-33Biosynthesis of coenzyme A from pantothenate, its vitamin precursor. Page 1101