Bio 261 September 30, 2003 Todd Vision Genetic Mapping “Speciation QTL” in Mimulus From QTL to an understanding the evolution of species differences at.

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Presentation transcript:

Bio 261 September 30, 2003 Todd Vision Genetic Mapping “Speciation QTL” in Mimulus From QTL to an understanding the evolution of species differences at a molecular level

QTL mapping

variation in leaf-level water use efficiency in rice McCouch et al., unpublished

rice (Kasalath x Nipponbare) WUE QTL

QTL mapping in Arabidopsis Hoekenga et al. (2003) Plant Physiol. 132: QTL map for root length at day 6, in liquid medium containing Aluminum

Arabidopsis Al-tolerance QTL both affect malate release

-What are the loci underlying speciation and species differentiation? - How did they arise? - What forces have shaped them? the genus Mimulus

Hybrid sterility in M. guttatus vs. nasutus Minor QTL –Differences in floral morphology Major QTL –a pair of epistatically interacting nuclear loci that cause complete pollen sterility in 1/8 of the hybrid F2 offspring –A cytonuclear incompatibility system causes complete male sterility in 1/4 of offspring carrying the M. guttatus cytoplasm M. nasutus growing in a mixed clump with M. guttatus in Shirley Creek, Calaveras Co, California. The red lines indicate M. nasutus flowers (photo: Mark MacNair)

Isolation via pollinators and habitat in M. lewisii vs. cardinalis Two major QTL underlying differences in pollination syndrome have been mapped –Introgression of the anthocyanin QTL (yup) from M. cardinalis into M. lewisii reduces bee visitation by 80%. –Allelic substitution from M. lewisii into M. cardinalis at the major nectar QTL (NEC1) reduces hummingbird visitation by 50%. lewisii F1 cardinalis F2

From QTL to gene QTL intervals contain many genes To get to causative polymorphisms, these things help: –Nearly isogenic lines (to Mendelize QTL) –Expressed sequence tags (cDNA sequences) –Large collections of markers (SSR, STS) –Comparative maps (e.g. to tomato & Arabidopsis) –Large-insert clones (BAC libraries) –Transgenics (to confirm the mapping results)

Duke Univ. John Willis Fred Dietrich Univ. of Washington Toby Bradshaw CUGI Jeffery Tomkins UNC Chapel Hill Todd Vision Michigan State Univ. Doug Schemske Univ. of Montana Lila Fishman

comparative maps Livingstone et al 1999 Genetics 152:1183 Gale & Devos 1998 PNAS 95:1972 Lycopersicon (tomato) vs. Capsicum (pepper)

Molecular footprint of selection Neutrality Population bottleneck Selection near locus 2 locus Schlotterer (2003) TIG 19, 32

Genomic technology Molecular evolution and population genetics Field ecology A rich and tractable biological system Molecular genetic architecture of species differentiation