AWACSS (EVK1-CT )IFAT 2002, Munich AWACSS Contract #: EVK1-CT Duration: 3 Year, RTD Project (Starting March, 2001) Visit: Automated Water Analyser Computer Supported System

AWACSS (EVK1-CT )IFAT 2002, Munich Goal: Develop a cost-effective, on-line, water monitoring biosensor that will measure a variety of small organic pollutants in short-time with remote control and surveillance. Expanded multi-analyte analysis capability allowing for up to 32 simultaneous analyte measurements. Immunochemical reagents are being developed for a host of small organic water pollutants, such as, Atrazine, Irgarol, Propanil, Caffeine, and Ethylhexlphthalate. Novel design approaches to the optical detection and fluidics including miniturized integrated optics and microfluidics. Limits of detection will match EU prescribed requirements. Reagent volumes will be reduced. Intelligent remote surveillance and control that will allow for unattended continuous monitoring. Instruments will be rugged and easy to handle by untrained personnel.

AWACSS (EVK1-CT )IFAT 2002, Munich Methodology: Immunoassay coupled with Total Internal Reflection Fluorescence laser waveguide PDLock-In filter PC absorbing coating bevelled polished end-face samples waste polymer fibres inject Analytederivative Analyte Antibody Fluorophor Analytederivative Analyte Antibody Fluorophor Analyte Antibody Fluorophore * * Flow Transducer * * Flow Remaining free antibodies are captured and detected. * * Flow * * Samples are pre-incubated with labelled antibodies.

AWACSS (EVK1-CT )IFAT 2002, Munich AWACSS Setup 2D Array Detection Miniaturisation Time Resolution Multi-analyte Detection 2D Array Detection 32 Analysis Spots Microfluidics Integrated Optics

AWACSS (EVK1-CT )IFAT 2002, Munich Networking Capabilites for Surveillance and Early Warning Monitoring of Industrial Plants  Contamination Monitoring of Side Streams  Origin of Pollution Monitoring of Coasts  Direction of Pollution Monitoring of Estuaries  Origin of Pollution

AWACSS (EVK1-CT )IFAT 2002, Munich Local and Global Intelligence Measurement Stations Local Intelligence Monitoring Data Analysis Control Station Global Intelligence Monitoring Data Analysis Data Recording Analysed Data Alerts Status Information Call for Measurements Manual Device Control Communication via the Internet

AWACSS (EVK1-CT )IFAT 2002, Munich Results and Progress to Date A prototype Integrated-Optical Chip waveguide to conduct light to 32 discrete analysis windows has been largely realised. The microfluidic flowcell has been designed and preliminarily tested with flow tests and mathematical flow simulations. The instrument hardware components have been selected and there connections and housings designed. Polyclonal antibodies directed against a variety of small organic water contaminants have been isolated and their corresponding analyte derivatives synthesised. A surface spotting procedure has also been developed. The water monitoring groups have catalogued contaminant levels at various European water sites and addressed water matrix effects relating to the AWACSS immunoassay chemistry. The first phase of a market survey has been conducted addressing relevant markets. A multi-media tutorial has been constructed that allows quick, low-cost training of the instrument's fundamental chemistry, software, and operation.

AWACSS (EVK1-CT )IFAT 2002, Munich Partners Eberhard-Karls-University Tübingen: Project Management and Surface Immunoassay Development Central Research Laboratory: Instrument Hardward Design Siemens: Electronics, Internet Communication, and Market Analysis. Optoelectronics Research Centre: Integrated Optical Chips Environmental Institute, Kos: Instrument Design and Field Tests- Slovakia King‘s College London: Immunochemsitry Institute of Chem. & Environ. Chem. IIQAB: Instrument Design and Field Tests-Spain DVGW-Technologiezentrum Wasser: Instrument Design and Field Tests- Germany Funding: This Project is funded under the 5th Framework Programme: Energy, Environment, and Sustainable Development. (1.7 million € contribution from the European Community.)