Chapter 1. Speed of Analysis (Technology) Power of Discrimination (Genetics) Low High SlowFast Markers Used (Biology) RFLP Single Locus Probes RFLP Multi-Locus.

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Presentation transcript:

Chapter 1

Speed of Analysis (Technology) Power of Discrimination (Genetics) Low High SlowFast Markers Used (Biology) RFLP Single Locus Probes RFLP Multi-Locus Probes ABO blood groups Multiplex STRs DQ  single STR D1S80 mtDNA PolyMarker Figure 1.1, J.M. Butler (2005) Forensic DNA Typing, 2 nd Edition © 2005 Elsevier Academic Press

Sample Obtained from Crime Scene or Paternity Investigation DNA Extraction DNA Extraction DNA Quantitation DNA Quantitation PCR Amplification of Multiple STR markers PCR Amplification of Multiple STR markers Biology Separation and Detection of PCR Products (STR Alleles) Technology Sample Genotype Determination Genetics Comparison of Sample Genotype to Other Sample Results If match occurs, comparison of DNA profile to population databases Generation of Case Report with Probability of Random Match Figure 1.2, J.M. Butler (2005) Forensic DNA Typing, 2 nd Edition © 2005 Elsevier Academic Press

gender ID A B C D E F G H I J gender ID A B C D E F G H I J Figure 1.3, J.M. Butler (2005) Forensic DNA Typing, 2 nd Edition © 2005 Elsevier Academic Press

Lessons from the First Case Involving DNA Testing Describes the first use of DNA (in 1986) to solve a double rape-homicide case in England; about 5,000 men asked to give blood or saliva to compare to crime stains Connection of two crimes (1983 and 1986) Use of DNA database to screen for perpetrator (DNA only done on 10% with same blood type as perpetrator) Exoneration of an innocent suspect DNA was an investigative tool – did not solve the case by itself (confession of accomplice) A local baker, Colin Pitchfork, was arrested and his DNA profile matched with the semen from both murders. In 1988 he was sentenced to life for the two murders.

Historical Perspective on DNA Typing Capillary electrophoresis of STRs first described First STRs developed FSS Quadruplex First commercial fluorescent STR multiplexes CODIS loci defined STR typing with CE is fairly routine Identifiler 5-dye kit and ABI 3100 PCR developed UK National Database launched (April 10, 1995) PowerPlex ® 16 (16 loci in single amp) 2005 : DNA is an important part of the criminal justice system Y-STRs Justice for All Act ($1B over 5 years) RFLP DQA1 & PM (dot blot) Multiplex STRs mtDNA