Masaryk University Enantis, s.r.o. VOP-026 Šternberk, s.p., division VTÚO Brno CPN, s.r.o. E N Z Y M A T I C D E C O N T A M I N A T I O N O F S U L F U R M U S T A R D

W a r f a r e a g e n t s u l f u r m u s t a r d  used for the first time in 1917 near Belgium city Ypres  easily penetrates to various materials  makes burn marks and blisters due to skin contacts  military use banned by international convention  easy to prepare from common chemicals

T e c h n o l o g y d e v e l o p m e n t  Czech Republic – development of enzymatic decontamination mixture for sulfur mustard prioritized by NATO  research projects funded by Ministry of Defence 2004 – 2006 and Ministry of Education 2005 – 2011  degradation of sulfur mustard by enzymes dehalogenases demonstrated  protection of intellectual properties – Czech patent CZ  technology transfer to practice – biotechnology spin-off Enantis

H a l o a l k a n e d e h a l o g e n a s e s

T o x i c s u l f u r m u s t a r d S Cl

O Cll N o n t o x i c a n a l o g u e

C o m p u t e r m o d e l l i n g

bis(2-chloroethyl) ether sulfur mustard C o m p u t e r m o d e l l i n g

E x p e r i m e n t a l t e s t s

P a t e n t - p r o t e c t e d c o n c e p t

R e p o r t s i n s c i e n t i f i c j o u r n a l s

T e c h n o l o g y t r a n s f e r t o p r a c t i s e 1.development of decontamination mixture 2.optimisation of protein production 3.large-scale industrial production 4.product commercialization 5.development of new and improved products

P r o d u c t d e v e l o p m e n t a n d t e s t i n g  testing of dehalogenases in decontamination aerosols (Pacific Northwest National Laboratory – USA)  testing of dehalogenases in polymer particles (Foster & Miller, US Army – USA)  testing of dehalogenases in microemulsions (Defence Science and Technology Laboratory – Great Britain)  testing of dehalogenases in skin decontamination cream (Bundeswehr Institute of toxicology and pharmacy – Germany)  NATO testing of enzyme decontamination on aircraft interriers

C o n s o r t i u m o f p a r t n e r s Masaryk University and Enantis, s.r.o.  14 years of research on dehalogenases  > 40 genes, >100 protein variants  know-how from 29 research stages  modern laboratories and facilities for protein engineering E N Z Y M E D I S C O V E R Y A N D O P T I M I Z A T I O N  isolation and construction of new enzymes  genetic breeding of enzymes with improved catalytic properties

C o n s o r t i u m o f p a r t n e r s Masaryk University and Enantis, s.r.o. VOP-026 Šternberk, s.p., division VTÚO Brno  applied research and development of decontaminations and defence against warfare of mass destruction  personal and infrastruture based on 50 years of experience T E S T I N G O F E N Z Y M E S W I T H W A R F A R E A G E N T S  study of warfare chemicals with extreme toxic properties  compatibility testing of individual components for decontamination mixtures

C o n s o r t i u m o f p a r t n e r s Masaryk University and Enantis, s.r.o. VOP-026 Šternberk, s.p., division VTÚO Brno CPN, s.r.o.  Production of pharmaceuticals and cosmetics  large-scale industrial fermentations and down-stream processing P R O C E S S S C A L E – U P A N D P R O D U C T I O N  optimization of enzyme production, pilot-scale and full production  development of protective cream for skin decontamination

E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r  high-quality basic research:  systematic and selective financial support  investment into human resources  competitive infrastructure  financial support and consulting for protection of intellectual properties  business consulting and administrative support  collaboration with local industrial partners

E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r  high-quality basic research:  systematic and selective financial support  investment into human resources  competitive infrastructure  financial support and consulting for protection of intellectual properties  business consulting and administrative support  collaboration with local industrial partners

E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r  high-quality basic research:  systematic and selective financial support  investment into human resources  competitive infrastructure  financial support and consulting for protection of intellectual properties  business consulting and administrative support  collaboration with local industrial partners

A c k n o w l e d g e m e n t s CTT

Jan Josef Loschmidt (1821 – 1895) E x c u r s i o n t o l a b o r a t o r i e s

 established 1994, funded by industry, 1 laboratory  until today 18 grants, 6 international  laboratories 5, instrumentation 1 mil EUR  in parallel 12 research projects  know-how from 29 research stages, 11 visitors  courses Bioinformatics and Biocatalysis E x c u r s i o n t o l a b o r a t o r i e s

3 doctors, 10 doctoral students, 5 diploma students, 1 technician