Principles and Practices of Biosafety Environmental Health and Safety San Diego State University

Abbreviations BSC – Biosafety Cabinet BSC – Biosafety Cabinet BSL – Biosafety Level BSL – Biosafety Level ABSL – Animal Biosafety Level ABSL – Animal Biosafety Level BSO – Biosafety Officer BSO – Biosafety Officer BUA – Biological Use Authorization BUA – Biological Use Authorization CA – California CA – California CDC – Center for Disease Control and Prevention CDC – Center for Disease Control and Prevention DGR – Dangerous Goods Regulations DGR – Dangerous Goods Regulations DOT – Department of Transportation DOT – Department of Transportation EHS – Environmental Health and Safety EHS – Environmental Health and Safety EPA – Environmental Protection Agency EPA – Environmental Protection Agency HMR – Hazardous Materials Regulations HMR – Hazardous Materials Regulations IACUC – Institutional Animal Care and Use Committee IATA – International Air Transport Association IBC – Institutional Biosafety Committee IRB – Institutional Review Board NIH – National Institute of Health OSHA – Occupational Health and Safety Administration PI – Principal Investigator PP – Physical Plant PPE – Personal Protective Equipment PS – Pubic Safety SD – San Diego USPS – U.S. Postal Service

Introduction The management of biological hazards through the proper application of engineered containment and administrative controls is referred to as biosafety or biohazard control. Biosafety or biohazard control is a team effort involving the PI, research lab personnel, BSO, IBC, IRB, IACUC, EHS, PP and PS.

Regulatory Requirements and Guidelines NIH Guidelines for Research Involving Recombinant DNA Molecules (April 2002) NIH Guidelines for Research Involving Recombinant DNA Molecules (April 2002) CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (May 1999) CDC/NIH Biosafety in Microbiological and Biomedical Laboratories (May 1999) CAL/OSHA Bloodborne Pathogen Standard CAL/OSHA Bloodborne Pathogen Standard CA Medical Waste Management Act CA Medical Waste Management Act DOT Transportation of Hazardous Materials DOT Transportation of Hazardous Materials

SDSU Biosafety Requirements and Guidelines Policies established by the Institutional Biosafety Committee (IBC) that meet or exceed applicable guidelines and regulations for use of RG 2 and RG 3 biohazardous materials or agents as well as non-exempt NIH Recombinant DNA research. Policies established by the Institutional Biosafety Committee (IBC) that meet or exceed applicable guidelines and regulations for use of RG 2 and RG 3 biohazardous materials or agents as well as non-exempt NIH Recombinant DNA research. Policies established by Environmental Health and Safety (EHS) that meet or exceed applicable regulations and guidelines for minimizing bloodborne pathogen exposure and disposal of biohazardous wastes. Policies established by Environmental Health and Safety (EHS) that meet or exceed applicable regulations and guidelines for minimizing bloodborne pathogen exposure and disposal of biohazardous wastes.

Biohazardous Material Infectious microorganisms (bacteria, viruses, fungi, parasites, prions, rickettsiae, etc.) affecting humans and animals Infectious microorganisms (bacteria, viruses, fungi, parasites, prions, rickettsiae, etc.) affecting humans and animals Diagnostic (clinical) specimens Diagnostic (clinical) specimens Recombinant DNA (viral vectors, gene therapy, cloning) Recombinant DNA (viral vectors, gene therapy, cloning) Genetically Modified Microorganisms (transgenic plants and animals) Genetically Modified Microorganisms (transgenic plants and animals) Human and non-human primate cells, cell culture (primary and immortalized), tissues, blood (whole blood or any components) and body fluids Animal or plant cells, cell cultures, fluids, tissues or derived wastes which may contain pathogens Animals known to be reservoirs of zoonotic disease