Biodegradation of the Prestige fuel oil under simulated conditions S. DÍEZ a, J. SABATÉ b, M. VIÑAS b, J. M. BAYONA a, A. M. SOLANAS b and J. ALBAIGÉS.

Slides:

Advertisements

Similar presentations

Chapter 20 – Organic Pollutants

Advertisements

Acute Effects Of Single And Mixed Polycyclic Aromatic Hydrocarbons Associated To Oil Spills On The Copepod Oithona Davisae. Carlos Barata 1, Albert Calbet.

Oil Cleanup Bacteria Richard Wolfe. Introduction A wide variety of micro-organisms are known to degrade petroleum. Batch culture A technique used to grow.

ENVIRONMENTAL ANALYSIS I. Solid Phase Extraction sorbent configurations – layered phases.

POLYCYCLIC AROMATIC HYDROCARBONS MONITORING IN TERRESTRIAL PLANT BIOTA AFTER ERIKA OIL SPILL N. Poupart, A. Meudec, F. Poncet, J. Dussauze Laboratoire.

BACTERIOPLANKTON DYNAMICS DURING A MESOCOSM-SIMULATED OIL SPILL Eva Teira 1, JM Gasol 2, I Lekunberri 2, XAG Morán 3, E Fernández 1, XA Álvarez-Salgado.

Air Sparging at Fort Greely, Alaska Presented by Aung Syn & James Powell.

Organic Chemistry CE 541. Aromatic Hydrocarbons They are hydrocarbons that include benzene and compounds containing aliphatic or aromatic groups attached.

Ch 16 Amines Homework problems: 16.9, 16.10, 16.21, 16.25, 16.39,

UNIT 10 CARBON CHEMISTRY QUÍMICA 1 BATXILLERAT. Isomerism Two chemical compounds are isomers when they have the same simplified molecular formula but.

Bioremediation of marine environment contaminated with petroleum hydrocarbon Richard Mannion Martin Casserly Martin Gallagher.

Field Study - Improved Grease Removal The Industrial Waste Treatment Business 44 75% odor removed 55% solids removed 8 days treatment 100% solids removed.

Spatial and temporal distribution of petroleum hydrocarbons in wild mussels in Galicia after the Prestige oil spill L. VIÑAS, M.A. FRANCO, J.A. SORIANO.

Carolina Science Supply.  The activities in this kit are intended to simulate how oil-degrading microbes can be used to break down petroleum after oceanic.

UPGRADING BIOMASS PYROLYSIS VAPOUR OVER FAUJASITE CATALYSTS T.S. NGUYEN, A. IMRAN, L. LEFFERTS, G. BREM, K. SESHAN.

TRIALS OF IN SITU BIOREMEDIATION ON THE BEACH OF SORRIZO (SPAIN) P. Fernández-Álvarez, J.M. Garrido and J.M. Lema Department of Chemical Engineering Universidade.

Variations in the V and Ni content in mussels after the Prestige spill VERTIMAR-2005 SYMPOSIUM ON MARINE ACCIDENTAL OIL SPILLS Vigo, Spain, July.

Nomenclature of Aromatic compounds  When a benzene ring is a substituent, the term phenyl is used (for C 6 H 5 - ) You may also see “Ph” or “  ” in place.

Bioremediation of PAHs-contaminated marsh soil by white-rot fungi Lara Valentín Carrera Chemical Engineering Department University of Santiago.

Dissolved/dispersed aromatic hydrocarbons in seawater in the area affected by the Prestige oil spill Lucia Viñas, María Ángeles Franco, Demetrio de Armas,

Oil Spills Jen Gollehon and Lindsey Poage Lindsey Poage.

Lecture 10 Ch Alkanes/Nomenclature

The Effects of Nitrogen Fertilization on Nutrient Cycling and Forest Productivity By: Eric Sucre.

Organic matter migration through sediments without water 4(v)

Biodiesel By: Ben Hobbs. Unit Objectives  What is biodiesel or diesel for that matter?  The chemistry of how biodiesel made?  How can it be used? 

Chung-Shiau Ho*,Wen-Yen Huang, Wen-Ling Hong, Chitsan Lin, Po-Han Chen

4A10 Construction Research & Innovation BioGeoChemistry Professor Mark Dyer TrinityHaus.

J. Zhou 1, X. Zhu 1, T. Wang 1, and X. Zhang 2 J. Zhou 1, X. Zhu 1, T. Wang 1, and X. Zhang 2 1 College of Resources and Information Tech., China University.

Kelly McFarlin, Mary Beth Leigh and Robert Perkins University of Alaska Fairbanks Indigenous Microorganisms Degrade Oil in Arctic Seawater Introduction.

The production of caseinophosphopeptides (CPPs) from a casein-derived by-product generated during the manufacture of an antihypertensive hydrolysate was.

To evaluate the potential chemo-protective effects of lunasin against tert-butyl hydroperoxide (t-BOOH) induced oxidative stress in human liver HepG2 cells.

Q. Lu 1, R.A. de Toledo 1, F. Xie 1, J. Li 1,2, H. Shim 1 1 Department of Civil and Environmental Engineering, Faculty of Science and Technology, University.

Cornell University 2009 ASA-CSSA-SSSA Meetings High C/N ratio Refugia pH & aeration Physico- chemical sorption Surface change Microbes Nutrients Amending.

By Dr. Nahed Nasser Carboxylic Acids and Their Derivatives.

Biodegradation of MTBE and BTEX in a Full-Scale Reactor down to ppb Levels after Inoculation with a MTBE Degrading Culture Erik Arvin, DTU Environment.

Colonization, biofilm formation and biodegradation of polyethylene by soil bacteria Alex Sivan The Institute for Applied Biosciences and The Department.

Chemistry 30 Organic Chemistry NOTES. I. Organic Chemistry Definition Organic compounds are those obtained from living organisms. Inorganic compounds.

Field Application of a Genetically Engineered Microorganism for Polycyclic Aromatic Hydrocarbon Bioremediation Process Monitoring and Control.

Bioremediation-From the Lab to the Field

Pete the penguin says… “Before “Before the show begins, don’t forget to visit the snack bar”

Chang Am L&S Co. LTD., 1 환경친화적 윤활제의 개발 및 동향 Developments & Trends of Environmental Friendly Lubricants 조원오 *, 김영운 **, 정근우 ** *

Natural resources have a high potential in solving current and future problems. During the last ten years our research group has studied the ability of.

“Selection of an actinobacteria consortium for enhancing diazinon degradation” Briceño Gabriela., Schalchli Heidi., Palma Graciela., Diez M. Cristina.

 Changing Environment  Urban Contaminants of Concern ◦ Heavy Metals ◦ Polycyclic Aromatic Hydrocarbons (PAHs)  In Situ Remediation ◦ Mechanisms ◦ Influencing.

Molecular Markers and Trace-Metals as Indication of Biomass Combustion in Central Amazon Sediments Tereza Cristina Oliveira Angela Wagener e Arthur Scofield.

指導教授 : 王振乾學生 : 陳雅貞日期： Abstract The biodegradation and hydrolysis rates of an aliphatic aromatic copolyester(PBAT) were measured in manure,food,

STORMWATER SAMPLING OF OIL PRODUCTS USING SEMIPERMEABLE MEMBRANE DEVICES (SPMDs) Per-Anders Bergqvist, *Lina Ulčinienė, *Viktoras Račys and *Audronė Žaliauskienė.

Organo-Sulfur and Receptor Modeling Status/Challenges Christopher Palmer Department of Chemistry and Biochemistry.

Biofuels Spring Turning Chemical Energy into Mechanical Work C 8 H 18 (l) + 25/2 O 2 (g)  8 CO 2 (g) + 9 H 2 O(g) + energy.

學生 : 陳雅貞日期 : Introduction Polycyclic aromatic hydrocarbons (PAHs) are pollutants of major concern in soils and sediments. Surfactant-enhanced.

APPLICATIONS FOR MICROBIAL EXTRACTS TO ADDRESS CLIMATE CHANGE CHALLENGES 3 March

Understanding the fate of methylated PAHs in the terrestrial environment and groundwater. Project – QUB 10/06/09 Andrew Frazer Project supervisors-:

NE: No effect. Data were not significantly different from control. (p

Chapter 20 – Organic Pollutants

Tasman Spirit oil Tanker in 2003

Analysis of Low Concentrations of PAH and Alkyl PAH

Biodiesel Production from Recycled Vegetable Oil

Biodiesel Seminar On Submitted To: Submitted By:

Bioremediation Dr. Bhavesh Patel (M.Sc., Ph.D.) Principal

Table 4. COMBINATION THERAPY

Aromatic hydrocarbons

Comparative simulative studies using PHREEQC-Interactive and Visual MINTEQ model for understanding metal-NOM complexation occurring in cooling and raw.

Some Aspects of the Combustion of Heavy Fuel Oil Containing High Asphaltene Levels F.A. Atiku A.R. Lea-Langton, K.D Bartle, P.Biller, J.M. Jones, A.B Ross.

Biodiesel A Sustainable Fuel.

Acute Effects Of Single And Mixed Polycyclic Aromatic Hydrocarbons Associated To Oil Spills On The Copepod Oithona Davisae . Carlos Barata1, Albert Calbet.

Solution medium effects on the photolysis of polyaromatic hydrocarbons (PAHs) in aqueous medium Catherine D. Clark , Warren J. De Bruyn, Chapman University,

Aromatic – Aliphatic compounds ( Arenes )

Harry Behzadi, Ph.D VP of Business Development North America

Changing the structure of resin-asphaltenes molecules in cracking

Programme INTERREG III : STARDUST

Presentation transcript:

Biodegradation of the Prestige fuel oil under simulated conditions S. DÍEZ a, J. SABATÉ b, M. VIÑAS b, J. M. BAYONA a, A. M. SOLANAS b and J. ALBAIGÉS a a Instituto Investigaciones Químicas y Ambientales de Barcelona-CSIC. B Universidad de Barcelona. Departamento de Microbiología

The fate of spilled oil in the marine environment Fuente: Albaigés 1989

OBJECTIVES To evaluate the biodegradation extent of Prestige fuel oil in vitro experiments times by two consortia. –Aliphatics, aromatics and molecular markers. Effect of biodegradation on: –source and weathering indices To assess weathering effects on biodegradation. To evaluate the effect of oleophilic nutrients.

METHODOLOGY

Microbial Consortia Enrichment AMTD Inocula: crude oil contaminated sand + 5-PAHs ( 3-4 aromatic ring) Inocula: diesel contaminated Soil + diesel oil Mineral medium 5 years

Biodegradation Experiments S200 : microemulsion urea + oleic acid + phosphate ester +2-butoxy-1-ethanol AM 50 mg AM 50 mg TD 50 mg original fuel weathered fuelfuel + S200 C h e m i c a l a n a l y s i s 20 days 40 days AM 50 mg TD 50 mg TD 50 mg original fuel 50 mg C h e m i c a l a n a l y s i s

Chemical Analysis Incubated fuel pH < 2.5 LLE (CH 2 Cl 2 ) organic extract Fractionation SPE (SiO 2 -C 3 CN) Aliphatics GC-FID GC-MS Aromatics GC-MS surrogates

HYDROCARBON BIODEGRADATION

Biodegradation Aliphatics

Time (min) N N1 N2 N3 P P1 P2 P3 Biodegradation Aromatics original fuel biodegraded fuel 20 days

Weathering Effect

Strain Efficiency

Oleophilic Nutrient Effect

(II) (I) m/z 106 m/z 92 (I) (II) m/z 106 alkyltoluenes m/z 92 alkylbenzenes Alkylbenzenes ORIGINAL FUEL BIODEGRADED (TD) 1-methyl-3-phytanylbenzene phytanylbenzene

C 2 & C 3 -Phenanthrenes ORIGINAL BIODEGRADED ORIGINAL BIODEGRADED m/z 206 m/z 220 C 2 -Phenanthrenes (P2) C 3 -Phenanthrenes (P3)

C 2 & C 3 - Dibenzothiophenes ORIGINAL BIODEGRADED ORIGINAL BIODEGRADED m/z 212 m/z 226 C 2 - Dibenzothiophenes (D2) C 3 - Dibenzothiophenes (D3)

Source Indices Hopanes Steranes

BIODEGRADATION MOLECULAR MARKERS Acyclic isoprenoids Diasteranes C 27 -steranes  -Steranes Homohopanes Monoaroamtic steranes Triaromatic steranes biodegradation

Effect of Biodegradation on Source Diagnostic Ratios IndexOriginal oil Biodegraded oil AM 20 days AM+S days Pr/Ph %23  %24TT11 7 %27Ts %29  %27d %29  S 4549 %29  %27  37 7 hopanes steranes isoprenoides

Source Indices D2/P2 D3/P3

Effect of Biodegradation on Source Diagnostic Ratios IndexOriginal oil Biodegraded oil AM 20 days AM+S days D2/P D3/P

Weathering Indices D2 D3 D2/C2 D3/C C2 C3

Effect of Biodegradation on Weathering Diagnostic Ratios IndexOriginal oil Biodegraded oil AM 20 days AM+S days D2/C D3/C370 51

N-alkanes, alkylbenzenes low MW parent PAHs and mono and di-alkylated derivatives are degraded by both consortia in days incubation. Degradation rates of PAHs decrease according with the alkyl substitution and number aromatic rings. Weathering slow down biodegradation. Oleophilic nutrient enhance the biodegradation of the more refractory PAHs and some molecular markers. Source indicators can be modified when oleophilic nutrient are added to the incubation medium. CONCLUSIONS

ACKNOWLEDGEMENTS Financial support was obtained from the MCYT (VEM CO5-01). Dr. S. Diez acknowledges a Ramon & Cajal contract.