Molecular Biology Lecture 23 Chapter 20 DNA repair Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.

Fig

Fig

Fig

Fig

Excision repair Base excision repair: Most prevalent and works on subtle changes in DNA structure caused by chemical modifications Nucleotide excision repair: Deals with more drastic changes to bases causing distortion of the DNA double helix

Fig Base excision repair

Fig Nucleotide excision repair base long

Fig Mismatch repair

Fig Recombination repair

Fig Error prone (SOS) bypass

List of problems to review before the final examination Recombinant DNA technology Southern blot RT-PCR Plasmids and host strains PCR for cloning Site-directed mutagenesis Restriction mapping Gene regulation in prokaryotes LAC operon TRP operon R-loop hybridization experiments Gene regulation in eukaryotes Regulation of GAL genes in yeast Post-transcriptional control (Tr receptor and ferritin) Chromatin structure and gene expression (figure and 13.22) DNA replication Mechanism and proteins participating in DNA replication