SS -1 PAC 8/18/11 Recoding the Genome Peter Carr 18 August 2011 FAB7 Lima, Peru.

Slides:



Advertisements
Similar presentations
Hoover High School Mr.Plazaks Biology : Write an answer here What is the sequence of an RNA molecule produced from transcription of the following DNA.
Advertisements

3 Types of RNA.
Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement Isaacs, Farren J. et al. Science, Vol 333, 2011 Presented by: PJ Velez.
Points to Ponder What are three functions of DNA?
KEY CONCEPT Mutations are changes in DNA that may or may not affect observable traits/characteristics.
Do Now:.  TRANSCRIPTION: process that makes an RNA copy of DNA.  RNA is single-stranded, and T is replaced by U (A-U; G-C)  RNA polymerase makes RNA,
Recombinant DNA technology
The DNA Bead String Exercise Anita L DeStefano, PhD Department of Biostatistics BU School of Public Health Co-Director Biostatistics Program Associate.
Simon Howden Applied Evolution Why Evolution Matters Belize Evolution Education Workshops, Nov
KEY WORDS – CELLS, DNA, INFORMATION All living things are made from Deoxyribonucleic acid is abbreviated This molecule stores that helps cells carry.
Chassis Parts jukebox : Meta-device : few strains Focus of system designs & integration Standardization, composability, abstraction Interface with environment,
2.7 DNA Replication, transcription and translation
14.4 Gene Mutations. What is a Mutation? A mutation is any change in the amount or structure of the DNA of an organism. KEY POINT: If this occurs in somatic.
Error-Corrected DNA Synthesis Peter Carr MIT Media Laboratory.
Biotechnology VC Funding, Vaccines, Technology. What is Biotechnology? Biotech: refers to the use of living organisms or their products to modify human.
DNA’s Function. DNA DNA = deoxyribonucleic acid. DNA carries the genetic information in the cell – i.e. it carries the instructions for making all the.
Copyright © 2007 Pearson Education Inc., publishing as Pearson Benjamin Cummings Organic Macromolecules and the Genetic Code A cell is mostly water. –The.
Chapter 13.2 (Pgs ): Ribosomes and Protein Synthesis
MAS.S62 FAB Synthetic Organisms and Novel Genetic Codes -Expanding the Genetic Code -Minimal Genomes -Genome Scale Engineering.
Rapid Prototyping for Biological Design Peter Carr CBA Bits  Biology 5/1/14 This work is sponsored by the Assistant Secretary of Defense for Research.
Molecular Biology Primer for CS and engineering students Alan Qi Jan. 10, 2008.
From Nucleotide Sequence to Amino Acid Sequence
DNA & RNA Protein Synthesis. DNA be.com/watch?v =4PKjF7OumYo.
What must DNA do? 1.Replicate to be passed on to the next generation 2.Store information 3.Undergo mutations to provide genetic diversity.
MIT Molecular Machines (Jacobson) Group - Next Generation DNA Synthesis HTGAA.
LECTURE PRESENTATIONS For CAMPBELL BIOLOGY, NINTH EDITION Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert.
Recombinant DNA technology
A gene is a particular sequence (a string) of nucleotides on a particular site of a chromosome. It is made up of combinations of A, T, C, and G. These.
 DNA is the blueprint for life – it contains your genetic information  The order of the bases in a segment of DNA (__________) codes for a particular.
DNA Replication to Transcription to Translation. DNA Replication Replication : DNA in the chromosomes is copied in the nucleus. DNA molecule is unzipped.
Regents Biology Nucleic acids: Information molecules.
Questions # 1 DNA carries the code for making proteins.
 DNA is the blueprint for life – it contains your genetic information  The order of the bases in a segment of DNA (GENE) codes for a particular protein;
Rewriting the Genetic Code BLI Biological Research 2013 Synthetic Biology Research Project Sejal Jain.
Microfluidics for Gene Fabrication Peter Carr & David Kong MIT Media Laboratory.
Gene Technology Chapter Basic Steps of Genetic Engineering Genetic Engineering – process of manipulating genes for practical purposes Genetic.
DNA and Translation Gene: section of DNA that creates a specific protein Approx 25,000 human genes Proteins are used to build cells and tissue Protein.
KEY CONCEPT Biotechnology relies on cutting DNA at specific places.
Why synthsize genes and genomes? minimal life -genome rewrite -genetic circuits -metabolic pathways -vaccines -gene therapy -individual.
Do Now: On the “Modeling DNA Transcription & Translation” handout, figure out the compimentary DNA sequence AND the mRNA sequence.
DNA Processes. Objectives Be able to explain the processes of DNA replication, transcription and translation
From Megabytes to Megabases Building a Fab to Enable Synthetic Biology
The Genetic Code. The DNA that makes up the human genome can be subdivided into information bytes called genes. Each gene encodes a unique protein that.
The Making of Proteins Using the Genetic Information Stored in DNA
B.E Pruitt & Jane J. Stein Chapter 9, part B Biotechnology and Recombinant DNA.
Chapter 1 Principles of Life
Gene Technologies and Human ApplicationsSection 1 Section 1: The Human Genome Preview Bellringer Key Ideas Secrets of the Human Genome Applications of.
Chapter 1 Principles of Life. All organisms Are composed of a common set of chemical components. Genetic information that uses a nearly universal code.
Notes: Human Genome (Right side page)
Biotechnology Kline FHS. What can biotechnology do? Reunite families? Identify a criminal? Find your baby daddy? Clone your pet that died? Make new vaccines?
THE ROLES OF DNA.
DNA Technology. Definitions Genetic engineering - process of altering genes to combining DNA from two or more organisms. Genetic engineering - process.
KEY CONCEPT Entire genomes are sequenced, studied, and compared.
MOLECULAR GENETICS Mutations Definition
Synthetic Biology: A Brief Survey
Types of Mutations.
The making of proteins for …..
Old News TRANSCRIPTION: process that makes an _______ ___________ of DNA. RNA is ________________, and ___ is replaced by ___ (A-U; G-C) RNA___________________.
DNA Test Review.
Nucleotide.
KEY CONCEPT Entire genomes are sequenced, studied, and compared.
KEY CONCEPT Entire genomes are sequenced, studied, and compared.
DNA Structure and Function
Biotechnology and Recombinant DNA
KEY CONCEPT Entire genomes are sequenced, studied, and compared.
KEY CONCEPT Entire genomes are sequenced, studied, and compared.
Section 20.4 Mutations and Genetic Variation
Part I. Introduction and Genetic Engineering
GENE TECHNOLOGY Chapter 13.
KEY CONCEPT Entire genomes are sequenced, studied, and compared.
Presentation transcript:

SS -1 PAC 8/18/11 Recoding the Genome Peter Carr 18 August 2011 FAB7 Lima, Peru

SS -2 PAC 8/18/11 DNA On-Demand rewrite genomes minimal life genetic circuits metabolic pathways individual genes assembly scaffolds vaccines gene therapy DNA base pairs

SS -3 PAC 8/18/11 Carr & Church (2009) Nature Biotech year nucleotide bonds organic chemistry biochemistry biology How big can we go?

SS -4 PAC 8/18/11 The basic genetic code ACGTACGT 4 letters ACGTACGT X ACGTACGT X AAA AAC AAG AAT ACA ACC ACG ACT words (codons) =

SS -5 PAC 8/18/11 Second Position

SS -6 PAC 8/18/11 By engineering new genetic codes we can: produce organisms immune to viruses make universal systems for “plug and play” with new amino acids new room for plug & play block exchange of genes, a “genetic firewall” rearrange to block function Second Position

SS -7 PAC 8/18/11 Multiplex Automated Genome Engineering (MAGE) “Find and replace” with the DNA of the genome

SS -8 PAC 8/18/11 Key features of MAGE: Multiplexed: can address many sites at once Targeted: modifies specific sequences; changes made can be specific or random Iterated: weak efficiency can be compensated by automating many cycles of modification Tunable: can control the range of diversity generated in a population Clean: no DNA scars, no resistance gene markers, etc.

SS -9 PAC 8/18/11 All 314 sites in the genome modified (across 32 strains)

SS -10 PAC 8/18/11 Genome Engineering base pairs to megabases

SS -11 PAC 8/18/11 Joseph Jacobson Bram Sterling* Chris Emig Sam Hwang George Church (HMS) Farren Isaacs Harris Wang Marc LaJoie Shuguang Zhang (MIT) Funding: NSF Thanks to:

SS -12 PAC 8/18/11 Atoms to bits...to atoms to bits receivertransmitter Box 1Box 2 me you it clinical + research data genetic counseling health statistics forensics basic research molecules experiments G AT T ACA

SS -13 PAC 8/18/11 Personal Box 1 MY GENES Box 2 How will drugs X, Y, Z affect ME ? MY TREATMENT How will MY BODY metabolize drugs X, Y, Z?

SS -14 PAC 8/18/11 Box 1 NEW PATHOGEN Box 2 Analyze the PARTS RAPID RESPONSE Test existing DRUGS at many sites Preliminary VACCINE Community

SS -15 PAC 8/18/11 MY IDEA Box 2 new biochemistry MY DESIGN FOR... DNA nanostructures new vaccines novel organisms Creativity

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2024 SlidePlayer.com. Inc. All rights reserved.