Reshma P Shetty, Drew Endy and Thomas F Knight Jr

 Background  Synthetic Biology  Terms  Method  Current Research  Discussion

 What is Synthetic Biology?  A Method  Milestones  Recombinant DNA  PCR(Polymerase Chain Reaction)  Automated Sequencing  Automated Construction  Standards  Abstractions -Write Read Last 30 years Synthetic Biology

 Systems  Devices  Parts  DNA

 Vector  Plasmid  Polymerase  Restriction enzyme  Ligase

 Goal  Standardize the BioBrick vectors’ assembly process  Maintain  Cloning site  Selection marker  High copy orgin  Terminators flanking the cloning site  Verification primer annealing sites  Process  Build new BioBrick vectors’ backbone  Standard assembly for new BioBrick

 Basic BioBrick

 Build new BioBrick vectors’ backbone

 Standard assembly for new BioBrick

 Check  Cloning site  Selection marker  High copy orgin  Terminators flanking the cloning site  Verification primer annealing sites

 More than 200 parts  Some Systems

 Wetware  Biological film  Digital counting machine  Interdisciplinary research  Computer aided design