By: C.J. Beneduce Maria Guirguis Amanda Rode Mr. Normand Dubois.

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Presentation transcript:

By: C.J. Beneduce Maria Guirguis Amanda Rode Mr. Normand Dubois

[Cyanomethyltriphenylphosphonium] + Bromide- (CMTPPB) Chemical Formula: C 20 H 17 BrNP Molecular Weight: Melting Point: 279 C o

CMTPB and DNA Minor Groove Energy= kcal/mol Gradient= Major Groove Energy= kcal/mol Gradient= CMTPPB located is the minor groove of a 12 base pair DNA CMTPPB interacting with a 4 base pair DNA. Energy= kcal/mol Gradient=

Cary Spectrophotometer Data Tables DNA Temperature (C o )Absorbance DNA and CMTPPB Temperature (C o) Absorbance

Cary Spectrophotometer Graphs

Electrophoresis From left to right: linearized DNA(E), linearized DNA and CMTPPB(EB), plasmid DNA(X), plasmid DNA and CMTPPB(XB), and ladder (L). Trail 1 E- Band #1= 25cm EB- Band #1= 25cm X- Band #1= 22cm Band #2= 24cm Band #3= 28cm XB- Band #1= 21cm Band #2= 29cm L- Band #1= 16cm Band #2= 20cm Band #3= 23cm Band #4= 28cm

Discussion The results indicated that CMTPPB may have bound with the DNA. The evidence for this was: A negative energy on HyperChem On the Cary Spectrophotometer, there was a shift in the melting curve of the DNA and CMTPPB sample, compared to the native DNA sample For future investigations: More trials Different concentrations of the compound of interest