Initiation of Replication *Strands must separate DnaA Helicases (DnaB and DnaC) SSBPs (Single Stranded Binding Proteins) DNA gyrase (topoisomerase)

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Presentation transcript:

Initiation of Replication *Strands must separate DnaA Helicases (DnaB and DnaC) SSBPs (Single Stranded Binding Proteins) DNA gyrase (topoisomerase)

*Primase Initiation of Replication

The sliding clamp

The two strands of a double helix are antiparallel Continuous DNA synthesis = leading strand Discontinuous DNA synthesis = lagging strand

DNA Synthesis

Completing the process

DNA Synthesis Eukaryotes DNA unwinds at a replication origin Two replication forks form Bidirectional synthesis of DNA Much more DNA; complexed with proteins Eukaryotes have much more DNA Yeast replicons Mammalian cells up to 25,000 Solution: Multiple replication origins Yeast 4X that of E. coli Drosophila 100X that of E. coli