To create a polycaprolactone mesh which enables cell activity and seeks to eventually provide an application in the field of tissue engineering toward biomimetic skin graft.
Spun 4 more meshes Visited Janelia Farm and used SEM Changed procedure slightly Created Timeline
All 15 wt. % solution using acetic acid as solvent 15 kV 15cm Used syringe pump (flow rate.02 ml/s) for first three meshes › Now using pipette to reduce beading
Mesh 1, Image a Magnification: µm x µm
Mesh 1, Image b Magnification: µm x µm
Mesh 2, Image a Magnification: µm x µm
Mesh 2, Image b Magnification: µm x µm
View SEM images of new PCL meshes › Visiting Janelia Farm on March 9 Begin varying voltage of pure PCL Create chitosan/PCL solution Begin cell work Updates › ED › wikipage
Akhyari, P., Kamiya, H., Haverich, A., Karck, M., & Lichtenberg, A. (2008). Myocardial tissue engineering: The extracellular matrix. European Journal of Cardio-Thoracic Surgery, 34, doi: /j.ejcts Bhardwaj, N. & Kundu, S. C. (2010). Electrospinning: A fascinating fiber fabrication technique. Biotechnology Advances, 28, doi: /j.biotechadv Chong, E.J., Phan, T.T., Lim, I.J., Zhang, Y.Z., Bay, B.H., Ramakrishna, S., & Lim, C.T. (2007). Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomaterialia, 3, doi: /j.actbio Geng, X., Kwon, O-H., & Jang, J. (2005). Electrospinning of chitosan dissolved in concentrated acetic acid solution. Biomaterials, 26, Han, J., Branford-White, C.J., & Zhu, L.M. (2010). Preparation of poly(є-caprolactone)/poly(trimethylene carbonate) blend nanofibers by electrospinning. Carbohydrate Polymers, 79, doi: /j.carbpol Homayoni, H., Ravandi, S.A.H., & Valizadeh, M. (2009). Electrospinning of chitosan nanofibers: Processing optimization. Carbohydrate Polymers, 77, Lowery, J.L., Datta, N., & Rutledge, G.C. (2010). Effect of fiber diameter, pore size and seeding method on growth of human dermal fibroblasts in electrospun poly(є-caprolactone) fibrous mats. Biomaterials, 31, doi: /j.biomaterials Nisbet, D.R., Forsythe, J.S., Shen, W., Finkelstein, D.I., & Horne, M.K. (2009). A review of the cellular response on electrospun nanofibers for tissue engineering. Journal of Biomaterials Application, 24, Pham, Q.P., Sharama, V., & Mikos, A.G. (2006). Electrospinning of polymeric nanofibers for tissue engineering applications: A review. Tissue Engineering, 12, Shevchenko, R.V., James, S.L., & James, S.E. (2010). A review of tissue-engineered skin bioconstructs available for skin reconstruction. Journal of the Royal Society Interface, 7, doi: /rsif Sill, T.J., & von Recum, H.A. (2008). Electrospinning: Applications in drug delivery and tissue engineering. Biomaterials, 29, doi: /j.biomaterials Woodruff, M.A., & Hutmacher, D.W. (in press). The return of a forgotten polymer- Polycaprolactone in the 21 st century. Progress in Polymer Science. doi: /j.progpolymsci