Iron-Reducing Bacteria Hopkins Microbiology Course Presented by Ying Lei 22 July 2010.

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Presentation transcript:

Iron-Reducing Bacteria Hopkins Microbiology Course Presented by Ying Lei 22 July 2010

Reduction Process 1.Fe 3+ only – ferrihydrite (FeOOH · 0.4H 2 O) – orange/red solid 2.Fe 3+ /Fe 2+ – magnetite (Fe 3 O 4 ) – black, ferromagnetic solid 3.Fe 2+ only – ferrous iron – black, non-magnetic solid

Redox Tower Reduced SpeciesOxidized SpeciesRedox Potential (E 0 ’) [CH 2 O]CO mV Fe 2+ αFe 2 O mV Fe 2+ αFeOOH-280 mV CH 4 CO mV H2SH2SSO mV Fe 2+ γFeOOH-100 mV Fe 2+ Fe(OH) 3 0 mV Fe 2+ Fe mV H2OH2OO2O mV CH 3 CH(OH)COO FeOOH + 4 H 2 O  3 HCO Fe OH - lactate C 0 iron (III) Fe 3+ CO 2 C 4+ iron (II) Fe 2+ Canfield et al, Aquatic Geomicrobiology (2005)

Enrichment Medium ComponentAmountPurpose basal marine mineral medium1 Lbasic salts sodium lactate4.5 g (30 mM)carbon source NaHCO 3 30 mL (30 mM)pH buffer iron (III) hydroxide30 mL (40 mM)ferric iron source 7-vitamin solution30 mLcofactors SL 101 mLtrace elements Se-Wo solution1 mLtrace elements Adjust to pH 7.2 in Pfennig bottles and incubate under anoxic conditions. Hopkins Microbiology Course (2010)

Sample Collection Canfield et al, Aquatic Geomicrobiology (2005)

Sample Collection

Successful Enrichments Marcus

Thank You All Instructors and Students Chris Marcus Blanca

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