INHALATION EXPOSURE LABORATORY EUGENE APPLEBAUM COLLEGE OF PHARMACY AND HEALTH SCIENCES WAYNE STATE UNIVERSITY CONTACT INFORMATION: DEEPAK BHALLA, PH.D., PROFESSOR PHONE: VANITHA BHOOPALAN, M.S., RESEARCH ASSISTANT PHONE:

RESEARCH TOPICS  NANOPARTICLE TOXICITY  NANOPARTICLE INTERACTION WITH OTHER INHALED POLLUTANTS: IN VIVO & IN VITRO  DRUG DELIVERY:  VOLUME MAY BE A LIMITING FACTOR  ALTERNATIVE – INTRATRACHEAL APPLICATION  THERAPEUTIC VALUE OF ANTIBODY-CONJUGATED NANOPARTICLES

OZONE CONC. DASIBI HUMIDITY AIROZONE AIR HYGRO- METER SAMPLING MODULE HUMIDIFIER AIR DRYER PURAFIL ACTIVATED CHARCOAL PARTICLE FILTER FL O W M ET E R PARTICLE FILTER NOSE-ONLY CHAMBERS FILTER SYSTEM EXHAUST OXYGENOXYGEN FL O W M ET E R OZONIZER TEMPERATURE OZONE EXPOSURE

OZONE EXPOSURE SYSTEM (NOSE-ONLY)

CHAMBERS FOR NOSE-ONLY EXPOSURE TO OZONE

OZONE, HUMIDITY AND TEMPERATURE MONITORS

SMOKE EXPOSURE

WALTON’S SMOKE MACHINE

SMOKE IN MIXING CHAMBER (2 SEC. PUFF)

HEAVY SMOKE IN MIXING CHAMBER

CIGARETTE SMOKE WHOLE-BODY EXPOSURE CHAMBER

FILTERED CIGARETTE NON - FILTERED CIGARETTE

SCIREQ SMOKE MACHINE

COMPUTER CONTROLLED SMOKING

SMOKING REGIMEN 1) Two second standard puff 2) A period of high flow during which the smoke is pushed downstream towards the animals. 3) A period of high flow during which the smoke is cleared out prior to the next puff. (1)(2)(3) Room Air Smoke

CIGARETTE SMOKE CONDENSATE (24.9mg/ml; 5/26/2011)