Gamma-proteobacteria in seawater Sidhartha Goyal Hyunsoo Na.

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Presentation transcript:

Gamma-proteobacteria in seawater Sidhartha Goyal Hyunsoo Na

Contents Introduction on (CARD-)FISH Procedures Results Discussion

Fluorescent in-situ hybridization Detection and localization - Unculturable bacteria - Specific mRNAs in tissue Capture live bacteria by targeting rRNA

CARD-FISH Cy3DAPI

Procedures 1.Sample preparation - Fixation and immobilization 2.Permeabilization & inactivation of endogenous peroxidases 3.Hybridization 4.CARD 5.Microscopy

Results – relative abundance Requires training! Can Beach sample

Results – cells/ ml at C1/M m Requires training! Can

Results – cells/ml at Hopkins beach Requires training! Can

Distribution of cells on the filter Cy3DAPI

Discussion ` Training - Distinguish between cells and p articles Probe design may be challenging Consortium analysis –Physical association of cells Community composition…? Cheap and quick!

Thank you