Evolutionary Considerations in Sexual Reproduction More Rotifer Strangeness MI615 Andrew J. Pierce Microbiology, Immunology and Molecular Genetics Graduate Center for Toxicology Markey Cancer Center University of Kentucky

Reassembly of shattered chromosomes in Deinococcus radiodurans. Nature Oct 5;443(7111): Epub 2006 Sep 27. Zahradka K, Slade D, Bailone A, Sommer S, Averbeck D, Petranovic M, Lindner AB, Radman M.

Reassembly of shattered chromosomes in Deinococcus radiodurans. Nature Oct 5;443(7111): Epub 2006 Sep 27. Zahradka K, Slade D, Bailone A, Sommer S, Averbeck D, Petranovic M, Lindner AB, Radman M. Not I digests Red: irradiated 7000 Gy γ-IR (!) (hours post-IR) Figure 1

Reassembly of shattered chromosomes in Deinococcus radiodurans. Nature Oct 5;443(7111): Epub 2006 Sep 27. Zahradka K, Slade D, Bailone A, Sommer S, Averbeck D, Petranovic M, Lindner AB, Radman M.

Reassembly of shattered chromosomes in Deinococcus radiodurans. Nature Oct 5;443(7111): Epub 2006 Sep 27. Zahradka K, Slade D, Bailone A, Sommer S, Averbeck D, Petranovic M, Lindner AB, Radman M. Figure 2 neg IR IR +3h IR +3h +UV IR +3h + BrdU IR +3h + BrdU +UV UV dose Grown in BrdURepaired in BrdU Reassembly of shattered chromosomes in Deinococcus radiodurans. Nature Oct 5;443(7111): Epub 2006 Sep 27. Zahradka K, Slade D, Bailone A, Sommer S, Averbeck D, Petranovic M, Lindner AB, Radman M.

Reassembly of shattered chromosomes in Deinococcus radiodurans. Nature Oct 5;443(7111): Epub 2006 Sep 27. Zahradka K, Slade D, Bailone A, Sommer S, Averbeck D, Petranovic M, Lindner AB, Radman M. Figure 3 NATIVE DENATURING ssDNA dsDNA

Reassembly of shattered chromosomes in Deinococcus radiodurans. Nature Oct 5;443(7111): Epub 2006 Sep 27. Zahradka K, Slade D, Bailone A, Sommer S, Averbeck D, Petranovic M, Lindner AB, Radman M. Figure 4

Extreme resistance of bdelloid rotifers to ionizing radiation. Proc Natl Acad Sci U S A Apr 1;105(13): Epub 2008 Mar 24. Gladyshev E, Meselson M.

Fertility: producing at least one active daughter Fecundity: percent hatched eggs Dose: 140 Gy / hr Monogonant Bdelloid Table 1 Extreme resistance of bdelloid rotifers to ionizing radiation. Proc Natl Acad Sci U S A Apr 1;105(13): Epub 2008 Mar 24. Gladyshev E, Meselson M.

Dose dependence of bdelloid and monogonont reproduction Parental Reproduction F1 Reproduction Diamonds: DSB / Mbp Circles: E. dilatata (monogonant) Squares: P. roseola (bdelloid) Triangles: A. vaga (bdelloid) Fertility: empty symbols Fecundity: filled symbols Figure 1 Extreme resistance of bdelloid rotifers to ionizing radiation. Proc Natl Acad Sci U S A Apr 1;105(13): Epub 2008 Mar 24. Gladyshev E, Meselson M.

DNA double-strand breakage by IR in A. vaga (bdelloid) Extreme resistance of bdelloid rotifers to ionizing radiation. Proc Natl Acad Sci U S A Apr 1;105(13): Epub 2008 Mar 24. Gladyshev E, Meselson M. Figure 2 Standard breakage: 5 x breaks / Mbp / Gy

Massive horizontal gene transfer in bdelloid rotifers. Science May 30;320(5880): Gladyshev EA, Meselson M, Arkhipova IR.

Massive horizontal gene transfer in bdelloid rotifers. Science May 30;320(5880): Gladyshev EA, Meselson M, Arkhipova IR.

Massive horizontal gene transfer in bdelloid rotifers. Science May 30;320(5880): Gladyshev EA, Meselson M, Arkhipova IR.

Massive horizontal gene transfer in bdelloid rotifers. Science May 30;320(5880): Gladyshev EA, Meselson M, Arkhipova IR.

Massive horizontal gene transfer in bdelloid rotifers. Science May 30;320(5880): Gladyshev EA, Meselson M, Arkhipova IR.