The Protestant Legend Presented by: Thao Cao Mentor: Dr. Hiro Nonogaki Seed Biology Laboratory The Protestant Legend

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Presentation transcript:

The Protestant Legend Presented by: Thao Cao Mentor: Dr. Hiro Nonogaki Seed Biology Laboratory The Protestant Legend

Food Genetic information Dispersal unit

heOrganism/Modeling_the_Organism.htm Broccoli Cauliflower Brussels Sprouts Brassicaceae Canola

Dry seed NF-YA9 NF-YA3 Dry seed High Low Expression

Down-regulation of NF-YA gene by microRNA is essential to maintain normal developmental program of seeds.

MicroRNA RISC

Wild type Control Mutant

Conditional expression Chemical Alcohol Heat Shock Chemical Induction

Gene of Interest Promoter 5XUAS Switch CsMV Promoter Chemical Induction (ligand: methoxyfenozide) Courtesy of Dr. Roger Beachy, Donald Danforth Plant Science Center VP16 ActivationGAL4 DNA BindingEcdysone Receptor Active

Promoter GUS Promoter GUS Uninduced Induced

Wild type AGE:GUS days after Imbibition

Uninduced Induced

-- ++ NF-YA3 mNF-YA3

Actin - + NF-YA9 Wild type NF-YA9 mNF-YA

Howard Hughes Medical Institute Dr. Kevin Ahern Dr. Hiro Nonogaki Louis Stokes Alliance for Minority Participation Natalya Goloviznina Theresa Nguyen Dr. Cristina Martinez-Andujar Mariko Nonogaki Satoru Yamagishi

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