Cell Separation Using Magnetic Beads

MiniMACS® Separator MS Column Magnet

MACS® Technology MACS Technology Used For Cell Separation Magnetic Activated Cells Sorting Yields Relatively High Purity Cell Populations 3 Components Involved MACS MicroBeads MACS Column MACS Separator

MACS® Technology MACS MicroBeads MACS Column 50 nm in size Biodegradable Conjugated to monoclonal antibodies MACS Column Generates strong magnetic field

Labeling Of Cells With Beads Monoclonal Ab

Magnetic Separation High Gradient Magnetic Field Trapped Labeled Cells

Magnetic Separation Cells Of Interest

Direct Labeling Advantages Disadvantages Fast Yields High Purity Cell Populations Disadvantages Binding of antibody to surface marker may activate cell Some cell populations may require multiple markers to be identified

Indirect Labeling

Indirect Labeling Useful in cases with dim surface marker expression Allows for amplification of signal Secondary antibody recognizes Biotin FITC Fc portion of primary antibody Streptavidin instead of secondary antibody Recognizes biotin on primary antibody

Depletion Strategy Unwanted Cells Are Magnetically Labelled And Depleted Useful In Following Cases Removal of Unwanted Cells No specific antibody is available for target cell Binding of antibody to target cell results in activations

Depletion Strategy

Depletion Strategy

Depletion Strategy

Protocol For Isolation Of CD4+ Cells

Protocol For Isolation Of CD4+ Cells

Protocol For Isolation Of CD4+ Cells 1. Prepare degased buffer