Corneal Repair and Immunosuppressive Effect After Intracameral Human Amniotic Membrane Mesenchymal Stem Cells (hAM-MSCs) Application Alejandro Navas, MD,

Slides:

Advertisements

Similar presentations

Mesenchymal Stem Cells Adopt a Myofibroblastic Phenotype in Culture: Implications for Cellular Cardiomyoplasty Melanie A. Ngo, Ryan H. Cunnington, Sunil.

Advertisements

Anterior Segment OCT Angle and Vault Analysis After Implantable Collamer Lens Implantation in Patients With High Myopia Arturo Ramirez-Miranda M.D. Alejandro.

The effects of pore architecture in silk fibroin scaffolds on the growth and differentiation of BMP7-expressing mesenchymal stem cells Yufeng. Zhang Ph.D.

Evaluation of Human-Derived Feeder Layers for Ex Vivo Cultivation of Corneal and Oral Epithelium for Ocular Surface Reconstruction No financial conflict.

Ex Vivo Expansion of Oral Mucosal Epithelial Stem Cells on Freeze Dried Amniotic Membrane for Potential Use in Ocular Surface Reconstruction Dina Kobtan.

ROYAN INTERNATIONAL TWIN CONGRESS,2014. Effect of Umbilical Cord Blood Derived Platelet-lysate on Fibroblast Proliferation and Activation of Smad Signaling.

Adipose rabbit mesenchymal stem cells for the treatment of the chronic scar tissue of the vocal cords Dr. Vasiliki E Kalodimou, Head of Flow Cytometry-Research.

P2Y2 Agonist Promotes Corneal Epithelial Wound Healing ID: Yong-Soo Byun, MD, PhD; Jongsoo Joo, MD; Sung A. Lim, MD; Woong-Joo Whang, MD; Choun-Ki.

The authors have no financial disclosures relevant to the topic.

Long-Term Follow-Up of Toric Intraocular Lens Implantation for Keratoconus Alejandro Navas, MD, MSc, Martha Jaimes, MD, Jesús Cabral, MD, Arturo Ramirez.

Verteporfin Photodynamic Therapy in Patients with Corneal Neovascularization Rodrigo Bolanos MD 1, Alexandra Pena MD 1, Guillermo Dewit MD 1, Judith Sandra.

Himanshu Matalia, MD1,2 Arokiaraj Vincent, MSc2 D Kamesh, MSc2

Purpose We studied the interdevice agreement of three systems in the different measurements of the corneal and anterior segment biometric parameters. The.

Coculture with Embryonic Stem Cells Improves Neural Differentiation of Adipose Tissue-Derived Stem Cells Arash Javeri MD, PhD, Masoumeh Fakhr Taha PhD,

Photodynamic Therapy with Verteporfin Combined with Ranibizumab for Corneal Neovascularization Alexandra Peña, MD 1 ; Alejandro Navas, MD, MSc 2 ; Enrique.

MESENCHYMAL STEM CELLS OR: HOW I LEARNED TO STOP WORRYING AND LOVE DIFFERENTIATION. Matthew Downs || 1/19/10.

Faeze. Introduction  Stem cell therapy is certainly a promising area for research. These have the ability to give rise to many specialize cells in on.

ADULT STEM CELL DR ANDUJAR LPGN RESEARCH SCIENTIST.

This presentation will provide a brief introduction into stem cell technology. I hope that you enjoy the lecture. There will be several quiz questions.

Accelerated Transepithelial CXL for keratoconus in pediatric population Enrique O. Graue-Hernández, Andrew Olivo-Payne, Lucero J Pedro-Aguilar, Arturo.

Figure 12, Greatly enhanced total calcium deposition on CAP modified nHA/Chitosan scaffold after 3 weeks of culture. Data are mean ±SEM; n=9. *p

The authors have no financial interest in the subject matter of this poster. The Effect of Topical Epigallocatechin Gallate (EGCG) Treatment on Murine.

Biotechnology Research Project by Anna Dong, Soojin Jeong, Reina Ooka -Stem Cells-

"Biological Safety of Stingless Bee Melipona Beecheii Honey on the Ocular Surface in Albino Rabbit Animal Model" Jesus Cabral MD Arturo Ramirez-Miranda.

New Antiinflammatory Strategy to Treat Murine Dry-Eye Disease

Changes of Ocular Surface in a Rabbit Model of Short Term Desiccating Stress Wei-Li Chen, MD, PhD Associate Professor, National Taiwan University Hospital.

Brian C.K. Au, M.D. University of Arizona – Department of Ophthalmology & University of Texas Medical Branch – Department of Ophthalmology ASCRS 2008 Annual.

Ten-year Clinicopathological Review Of Ocular Surface Squamous Neoplasia In An Ophthalmological Center In Mexico City Lucero Pedro-Aguilar, M. D. Alvarez-Melloni.

Ocular surface reconstruction with autologous mucosal membrane transplantation in limbal stem cell deficiency Elisabeth Macdonald Suzannah Drummond Kanna.

Shen Bin Journal Club. What is MSCs?  Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of.

H. Ueno, G. Ferrari, T. Hattori, T. Funaki, D.R. Saban, S.K. Chauhan, R. Dana The authors have no financial interest in the subject matter of this poster.

Eun Chul Kim, M.D., Man Soo Kim, M.D. Department of Ophthalmology & Visual Science, College of Medicine, Catholic University of Korea, Seoul, Korea The.

Quantification of Soluble Cytokines in Sjögren Disease, non Sjögren dry eye and Healthy Subjects M. Jaimes 1B, C. Santacruz-Valdes 1B, Y. Garfias 1A, T.

1 Non-contact Specular Microscopy for Evaluation of Corneal Endothelium in Early Fuchs’ Endothelial Corneal Dystrophy Jianyan Huang 1, MD, PhD; Tudor Tepelus.

Scheimpflug and OCT analysis of posterior keratoconus

Stem Cell Identification Nozad H. Stem cell workshop Stem cell Research center Tabriz medical university In The Name of God.

Epithelial Dendritic Cells and Subbasal Nerve Plexus in Infectious Keratitis: An In Vivo Confocal Microscopy Study. Andrea Cruzat, MD, Dimos Mantopoulos,

Diane Marinho, Cláudia Borowsky, Carlos Galia Ophthalmology Department - Hospital de Clínicas de Porto Alegre Federal University of Rio Grande do Sul -

Correlation of in-vivo confocal microscopy findings with clinical severity of aniridia related keratopathy. Mandal N 1 Shortt A.J. 1,2,3 1. Cornea and.

Invest. Ophthalmol. Vis. Sci ;49(10): doi: /iovs Figure Legend:

From: The Use of Human Mesenchymal Stem Cell–Derived Feeder Cells for the Cultivation of Transplantable Epithelial Sheets Invest. Ophthalmol. Vis. Sci..

Martha Jaimes Gutierrez M.D Alexandra Abdala Figuerola M.D.

Emu oil Stabilized Stemness in Adipose Tissue derived Stem Cells Khatereh Saei Arezoumand1*, Effat Alizadeh1, 2, Mohammad Esmaeilluo3, Yones Pilehvar-Soltanahmadi1,2,

From: Mesenchymal Stem Cells Ameliorate Experimental Autoimmune Uveoretinitis by Comprehensive Modulation of Systemic Autoimmunity Invest. Ophthalmol.

From: Mesenchymal Stem Cells Ameliorate Experimental Autoimmune Uveoretinitis by Comprehensive Modulation of Systemic Autoimmunity Invest. Ophthalmol.

Mesenchymal Stem Cells Ameliorate Podocyte Injury and Proteinuria in a Type 1 Diabetic Nephropathy Rat Model Shuai Wang, Yi Li, Jinghong Zhao, Jingbo.

Ezzatollah Fathi1, Raheleh Farahzadi 2, *, Najmeh Sheikhzadeh3

Neoplastic blood cells become pluripotent

Systemic Administration of Multipotent Mesenchymal Stromal Cells Reverts Hyperglycemia and Prevents Nephropathy in Type 1 Diabetic Mice Fernando E. Ezquer,

Glycoprotein Nonmelanoma Clone B Regulates the Crosstalk between Macrophages and Mesenchymal Stem Cells toward Wound Repair Bing Yu, Talib Alboslemy,

Mesenchymal Stem Cells Ameliorate Podocyte Injury and Proteinuria in a Type 1 Diabetic Nephropathy Rat Model Shuai Wang, Yi Li, Jinghong Zhao, Jingbo.

Volume 11, Issue 6, Pages (December 2012)

Restoration of Corneal Transparency by Mesenchymal Stem Cells

Volume 10, Issue 5, Pages (May 2012)

Use of Intravital Multi-Photon Microscopy to Study In Vivo Migratory Kinetics of Corneal Bone Marrow-Derived Cells Pedram Hamrah, M.D., Dimosthenis Mantopoulos,

Endovenous Administration of Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells Prevents Renal Failure in Diabetic Mice Fernando Ezquer, Marcelo.

Mesenchymal Stem Cells Arrest Intervertebral Disc Degeneration Through Chondrocytic Differentiation and Stimulation of Endogenous Cells Fan Yang, Victor.

Intranigral Transplantation of Epigenetically Induced BDNF-Secreting Human Mesenchymal Stem Cells: Implications for Cell-Based Therapies in Parkinson's.

Volume 4, Issue 3, Pages (March 2015)

Interaction of kindlin-3 and β2-integrins differentially regulates neutrophil recruitment and NET release in mice by Zhen Xu, Jiayi Cai, Juan Gao, Gilbert.

Increased Lipocalin-2 Contributes to the Pathogenesis of Psoriasis by Modulating Neutrophil Chemotaxis and Cytokine Secretion Shuai Shao, Tianyu Cao,

Volume 18, Issue 10, Pages (October 2010)

Volume 140, Issue 2, Pages e4 (February 2011)

Volume 20, Issue 11, Pages (November 2012)

Hematology Journal Club

Volume 4, Issue 3, Pages (March 2015)

Stem cell Basics.

Volume 23, Issue 1, Pages (January 2015)

Volume 26, Issue 1, Pages (January 2018)

Moutih Rafei, Elena Birman, Kathy Forner, Jacques Galipeau

Presentation transcript:

Corneal Repair and Immunosuppressive Effect After Intracameral Human Amniotic Membrane Mesenchymal Stem Cells (hAM-MSCs) Application Alejandro Navas, MD, MSc, Fátima S. Magaña- Guerrero, BSc, Pamela Martínez-Aboytes, MD, Rodrigo Bolanos, MD, Enrique O. Graue-Hernández, MD, MSc, Yonathan Garfias, MD, PhD Institute of Ophthalmology “Conde de Valenciana”, Mexico City, Mexico

Financial Disclosure -AN: Carl Zeiss Meditec, Consultant (A); Alcon Laboratories, Consultant (B) and STAAR Surgical, Consultant (B). -FSMG, PMA, RB, EOGH & YG: No financial interest or relationship to disclose. -Grants and support: Authors would like to thank National Council of Science and Technology (CONACYT #160286), and Institute of Ophthalmology “Conde de Valenciana” Patronage.

Purpose To evaluate the effect of human amniotic membrane mesenchymal stem cells (hAM- MSCs) over components of the innate immune system and their possible use in the regenerative ocular therapy. Methods Isolated hAM-MSCs were cultured in DMEM/F12 with 10% fetal bovine serum (FBS). hAM-MSCs were characterized using anti- CD105, CD73, CD44, CD29 and CD45 antibodies by flow cytometry.

Methods In vitro tri-linage differentiation in hepatocytes, chondrocytes and neurons was performed. The xenotrasplant of hAM-MSCs was intracamerally performed in a murine corneal burn model. The effect of hAM-MSCs on Neutrophil Extracellular Traps (NETs) were evaluated by flow microscopy.

Figure 1: 3-millimeter diameter chemical burns were made. Different severity models were used: 1 NaOH, R-OH 99.6% Figure 2: Simultaneous (immediately after injuries) intracameral injections of hAM-MSCs or BSS (sham) were performed.

Neutrophlis Extracellular Traps (NET’s) Figure 3: NETs assays showing the capability of hA-MSCs to inhibit traps formation.

Results Cells with fibroblastic morphology and the ability to generate fibroblast-colony forming units were obtained. The phenotype of the cells was CD105+ CD73+ CD44+ CD29+ and CD45-, which suggests that these cells were mesenchymal cells. Their ability to differentiate into hepatocytes, chondrocytes and neurons was confirmed by the acquisition of albumin, collagen-II and nestin proteins, respectively.

Figure 5: Flow citometry characterization: CD 105, C D29, CD 73 Positive (+) and CD 34, CD45, CD 44 Negative (-). Figure 6: hAM-MSc transdifferentiation assays. Collagen II (Insuline/chondrocytes) [A] and nestin (Tran retinoic acid/neurons [B]. A B Figure 4: Mesenchymal stem cells (hAM- MSCs) obtained from human amniotic membrane.

Results The xenotransplant of hAM-MSCs significantly improved the transparency of the murine damaged corneas and reduced the inflammatory infiltrate. On the other hand the interaction of peripheral blood polymorphonuclear cells (PBPMN) with the supernatant of hAM-MSCs decreased the NETs production.

Figure 7: NaOH = Sodium hydroxide 1N; BSS = Balanced Salt Solution; hA-MSCs = human Amniotic membrane-Mesenchymal Stem Cells. Burn murine model C57BL6 showing severe corneal vascularization, opacification and inflammation (A), an improvement in the intracameral hA-MSCs group was showed (B). Also severe cellular infiltration in the anterior chamber in the sham group (C) compared with mild reaction in the treated group (D). Fluoresence hA-MSCs marked are presented under fluoresence microscope (E,F). [DAPI nuclear counterstaining]. NaOH + BSS (Sham) NaOH + intracameral hA-MSCs

Figure 8: R-OH = 99.6% alcohol; BSS = Balanced Salt Solution; hA-MSCs = human Amniotic membrane- Mesenchymal Stem Cells. A less severe murine burn model with absolute alcohol was performed. More vascularization and opacification were found in the sham group (A) compared with the hA-MSCs treated group (B). H&E histological showed more inflammation and cellular infiltration in BSS (sham) gropu (C,E) compared with intracameral hA-MSCs treated group (D,F). R-OH + BSS (Sham) R-OH+ intracameral hA-MSCs

Conclusions These data suggest that human Amniotic membrane-Mesenchymal Stem Cells (hA- MSCs) could be applied in the ocular regenerative therapy as an immunosuppressor treatment in ocular and inflammatory diseases. References 1)Zeng W, Li Y, Zeng G, Yang B, Zhu Y. Transplantation with cultured stem cells derived from the human amniotic membrane for corneal alkali burns: an experimental study. Ann Clin Lab Sci 2014;44: )Ma Y, Xu Y, Xiao Z, Yang W, Zhang C, Song E, Du Y, Li L. Reconstruction of chemically burned rat corneal surface by bone marrow-derived human mesenchymal stem cells. Stem Cells 2006;24: )Reinshagen H, Auw-Haedrich C, Sorg RV, Boehringer D, Eberwein P, Schwartzkopff J, Sundmacher R, Reinhard T. Corneal surface reconstruction using adult mesenchymal stem cells in experimental limbal stem cell deficiency in rabbits. Acta Ophthalmol 2011;89: )Jiang TS, Cai L, Ji WY, Hui YN, Wang YS, Hu D, Zhu J. Reconstruction of the corneal epithelium with induced marrow mesenchymal stem cells in rats. Mol Vis 2010:16: )Rohaina CM, Then KY, Ng AM, Wan Abdul Halim WH, Zahidin AZ, Saim A, Idrus RB. Reconstruction of limbal stem cell deficient corneal surface with induced human bone marrow mesenchymal stem cells on amniotic membrane. Trans Res 2014;163: