Forensic Science Blood Typing Lab Activity T. Trimpe

Blood Typing Lab3/2/15 Key Question: How can blood typing be useful? Initial Thoughts:

Evidence 1 Try the 8 practice problems.

Evidence 2  dtypinggame/  Read the Tutorial (What is a blood type? How do you determine a patient’s blood type? How do you perform blood transfusions?) and take notes.  Play the quick game random patients or same patients  dtypes/preview.weml

A small pool of blood and a weapon was found near a garbage dumpster. After examining the area, the CSI on the scene discovered a body in the garbage dumpster and identified him as Earnest “One-Eyed” Earl. Earl had a wound to his chest that will be analyzed by the medical examiner. The CSI tested blood samples from the blood pool and the weapon at the crime scene. It was determined that it was human blood, but he needs to know the blood type to help identify if it was from the victim or the person who murdered him. He has come up with three suspects that either knew the victim or were seen in the area before the body was discovered. He would like to question them further while he waits for DNA test results. Follow your teacher’s directions to complete the lab. Remember to be careful to prevent cross-contamination of the blood samples! Image: Evidence 3 - The Crime Report

We will be determining blood types using Anti-A serum, Anti-B serum, and Anti-RH serum. Use the chart to help you determine the blood type of each sample and its Rh factor (+ or -). Clumping = + (Positive) No Clumping = - (Negative) DIRECTIONS: Step 1: Place three drops of blood onto the table and add three drops anti-A serum then mix using knife (clean knife afterwards). Write results on the worksheet. Step 2: Place three drops of blood onto the table and add three drops anti-B serum then mix using knife (clean knife afterwards). Write results on the worksheet. Step 3: Place three drops of blood onto the table and add three drops anti-RH serum then mix using knife (clean knife afterwards). Write results on the worksheet. Step 4: Move to next lab station repeat Steps 1-3 until all lab stations are done. Blood Typing Test Rh Serum - Clumping = Rh+ blood Type Reaction w/ Anti-A Serum Reaction w/ Anti-B Serum A +- B -+ AB ++ O --

Agglutination = Clumping = Positive Reaction Clumping = Positive Result A B Rh What is this person’s blood type? Answer: A+ Rh Serum - Clumping = Rh+ blood Type Reaction w/ Anti-A Serum Reaction w/ Anti-B Serum A +- B -+ AB ++ O --

What do your results show? What would you do next in the investigation? Conclusion - = No clumping + = Clumping Results Use + or – to show the test results for each sample and then determine the blood type.

Analysis Questions  What are universal donors?  Explain how you get your blood type.  What happens if both your parents don’t have the same type of blood?  Compare the averages per blood type found in Evidence 1 to the 3 suspects and victim. How do they compare?

Summary  What did you think about blood typing before?  What did you learn from your evidence about blood typing?  What are some further thoughts or questions you have about blood typing?

Reflection  What do your results show?  What should investigators do next?

Big Idea

Rate My New Knowledge About Blood Typing  0 – I don’t understand  1 – I have some knowledge  2 – I understand the concept well  3 – I can explain my knowledge to someone else