90- How can we make more insulin? V.1.2.2 How can we make more insulin? By Transforming Bacteria V.1.2.2.

Slides:



Advertisements
Similar presentations
Biotechnology Bacterial Transformation. Biotechnology Can Be Used to Treat Disease.
Advertisements

PARA-R Sequence RFP Expression Sequence Biotechnology Lab Program Laboratory Protocols by: Marty Ikkanda Powerpoint by: Anthony Daulo Kristi Schramm Pierce.
PARA-R Recombinant RFP Expression Sequence biotechnology lab program Laboratory Protocols by: Marty Ikkanda Powerpoint by: Anthony Daulo Pierce College,
Preparing an Overnight Culture of Escherichia coli
pARA-R Sequence LABS 2a and 4a RFP Expression Sequence
Abridged Genetic Engineering Pathway (Original “A” Sequence)
Ch. 5A: Transforming Bacteria with Recombinant Plasmids.
Structure & Function of DNA. DNA and RNA are nucleic acids that consist of long chains of nucleotides The nucleotides have three parts; 1.Phosphate 2.Nitrogen.
Exercise 17 Bio 112 Genetics Lab. There are four grain phenotypes in the above ear of corn: Purple & Starchy(A), Purple & Sweet(B), Yellow & Starchy(C)
Chapter 20 DNA Transformation A. P. Biology Mr. Knowles Liberty Senior High School.
PGLO™ Transformation. Central Framework of Molecular Biology DNA RNA ProteinTrait.
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College. Summer 2008 Workshop in Biology and Multimedia for High School Teachers.
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College. Summer 2008 Workshop in Biology and Multimedia for High School Teachers.
Genetic engineering ­ Genetic engineering: manipulating DNA or organisms to perform practical tasks or provide useful products We’re going to look at the.
Introduction: How to Clone a gene?
Bacterial Transformation RET Summer Overall Picture Bio-Rad pGLO Transformation Insertion of GFP gene into HB101 E. coli.
PGLO™ & GFP.
GFP Transformation Lab Images taken without permission from
Biotechnology Explorer Program Serious About Science Education.
pGLOTM Bacterial Transformation Courtesy BioRad Corporation
Pierce College, Woodland Hills
PARA-R Sequence RFP Expression Sequence Biotechnology Lab Program Laboratory Protocols by: Marty Ikkanda Powerpoint by: Anthony Daulo Kristi Schramm Pierce.
Introduction to pGLO lab Bacteria Transformation Please take these notes carefully. You do not need to write anything in RED.
BRIDGES  DNA ➔ RNA ➔ PROTEIN ➔ TRAIT Genotype Phenotype.
PGLO ™ & GFP. Central Framework of Molecular Biology DNA RNA ProteinTrait.
Overview Amgen Biotech Labs In this set of labs, students will:
The Arabinose Operon Gene Regulation. Why Gene Regulation? Developmental Changes Cell Specialization Adaptation to the environment Prevents creation of.
Transformation of E. coli with Green Fluorescent Protein (GFP)
Hey Kim, this site shows the kids everything! NY ‘09 use this for the prelab stuff!! Michael.Gregory/files/Bio%20100/Bio%
Genetic Engineering BSC 1010L Transformation of E. coli with Jellyfish GFP.
Transforming E. coli with a Recombinant Plasmid. What is biotechnology? Employs use of living organisms in technology and medicine Modifying living organisms.
Bacterial Transformation
Bioengineering Turning Genes on and off in Bacteria.
Restriction Analysis of pDRK and pGRN Original Plasmids
Bacterial Genetics & Transformation
Bacterial Plasmids Loops of DNA found in some bacteria
In the pGLO lab, we will: Use recombinant DNA Genetically engineer E. coli bacteria by inserting a plasmid Plate and grow bacteria Determine if the proteins.
Moving Green Fluorescent Protein
Genetic Engineering and Gene Regulation. What is genetic engineering? Manually adding new DNA to an organism, giving an organism traits it wouldn’t naturally.
Liquid cultures to Lysis: Movie Clip from Teachers domain Student Guide Lab 6.
GFP Transformation Lab
Genetic Transformation of Bacteria and Gene Regulation.
Transport Nucleus Cytoplasm Protein gene DNA mRNA The Cell:
GENETIC TRANSFORMATION. WHAT IS GENETIC TRANSFORMATION Genetic transformation is altering a cell by adding genetic information from an outside source.
AP Biology Plasmids  Small supplemental circles of DNA  ,000 base pairs  self-replicating  carry extra genes  2-30 genes  genes for antibiotic.
In the pGLO lab, we will: Use recombinant DNA Genetically engineer E. coli bacteria by inserting a plasmid Plate and grow bacteria Determine if the proteins.
PGLO Bacterial Genetics. E. Coli structure.
Bacterial Transformation. Chromosome? A long piece of DNA with many pieces of information on it, each piece is a set of directions for making a protein.
GFP Transformation Lab
pGLO™ Transformation and Purification of
Genetics of Bacteria Bacterial genome =.
Accelerated Biology Transformation Lab
Lab 5a Transformation of Escherichia coli with pARA-R
Biotechnology
Bacterial Transformation
Genetic Transformation of Bacteria and Gene Regulation
Bacterial Transformation
Gene Expression 1. Gene expression is the activation of a gene that results in transcription and the production of mRNA. Only a fraction of any cell’s.
PGLO Lab Purpose: To transform E. coli bacteria by adding plasmids that allow the bacteria to glow green under UV light in the presence of arabinose sugar.
PGLO Transformation.
Accelerated Biology Transformation Lab
Transport Nucleus Cytoplasm Protein gene DNA mRNA The Cell:
Introduction to the pGLO Lab
Biotechnology
pARA-R Sequence RFP Expression Sequence Biotechnology Lab Program
Biology II Study Guide for Unit Test on Operons and Transformation Lab 2013 You should be able to … 1. describe gene expression by explaining the following:
Abridged Genetic Engineering Pathway (Original “A” Sequence)
Gene Regulation pGLO Transformation.
Unit 5 Day 3 Lab Results.
GFP Transformation Lab
Presentation transcript:

90- How can we make more insulin? V.1.2.2

How can we make more insulin? By Transforming Bacteria V.1.2.2

“transformed cell” – cell has acquired new characteristics “characteristics” – due to the expression of incorporated foreign genetic material

AP Biology Green with envy?? Jelly fish “GFP” Transformed vertebrates

AP Biology Genetic engineering… grow bacteria harvest (purify) protein transformed bacteria plasmid gene from other organism + recombinant plasmid vector

Plasmids Small circles of DNA –carry extra genes 2-30 genes genes for antibiotic resistance Plasmids

AP Biology How can plasmids help us?  A way to get genes into bacteria easily  insert new gene into plasmid  insert plasmid into bacteria  bacteria now expresses new gene  bacteria make new protein + transformed bacteria gene from other organism plasmid cut DNA recombinant plasmid vector glue DNA

Plasmid of Interest Plasmid of interest Genes in Plasmid: Amp r- Gene for ampicillin (an antibiotic) resistance araC- Gene for araC protein which regulates GFP transcription GFP- Gene for green fluorescent protein pGLO ori Amp r GFP araC

The Transformation Process

preparing competent cells for transformation Lipid bilayer (inner) Lipid bilayer (outer) Peptidoglycan layer Adhesion zone Calcium ions

transforming Escherichia coli with pGLO Recombinant Plasmids Competent Cells pGLO

Calcium ions pGLO transforming Escherichia coli with pGLO Adhesion zone Lipid bilayer (inner) Lipid bilayer (outer) Peptidoglycan layer

GFP expression araC gene GFP Gene P BAD Transcription mRNA Translation araC protein

GFP expression P BAD araC protein araC gene araC protein prevents GFP transcription by causing a loop to form in the region of the gfp gene GFP Gene

GFP expression araC protein arabinose araC geneP BAD arabinose – araC protein complex RNA polymerase Arabinose – araC protein complex prevents DNA looping and helps to align RNA polymerase on the promoter site (P BAD ). mRNA Transcription Translation GFP (green fluorescent protein) GFP Gene

Work on pGLO Reading