BioFire (FilmArray) Multiplex PCR Assays Teresa Finken, MT(ASCP)

Panels Available Respiratory Viral Panel Blood Culture Identification Panel Gastrointestinal Panel

Methodology Multiplex Real-Time PCR Assay These panels test for multiple targets simultaneously This methodology is real-time PCR meaning that it amplifies and detects at the same time

What does this Mean to You? One sample One test Multiple results 1.5-hr turnaround time

Respiratory Viral Panel Targets Viruses Adenovirus Coronavirus HKU1 Coronavirus NL63 Coronavirus 229E Coronavirus OC43 Human Metapneumovirus Human Rhinovirus/Enterovirus Influenza A Influenza A/H1 Influenza A/H1-2009 Influenza A/H3tapneumovirus Influenza B Parainfluenza 1 Parainfluenza 2 Parainfluenza 3 Parainfluenza 4 Respiratory Syncytial Virus Bacteria Bordetella pertussis Chlamydophila pneumoniae Mycoplasma pneumoniae

Blood Culture Id Panel Targets Gram + Bacteria Enterococcus Listeria monocytogenes Staphylococcus Staphylococcus aureus Streptococcus Streptococcus agalactiae Streptococcus pyogenes Streptococcus pneumoniae Yeast Candida albicans Candida glabrata Candida krusei Candida parapsilosis Candida tropicalis Antibiotic Resistance mecA - methicillin resistant vanA/B - vancomycin resistant KPC - carbapenem resistant Gram – Bacteria Acinetobacter baumannii Haemophilus influenzae Neisseria meningitidis Pseudomonas aeruginosa Enterobacteriaceae Enterobacter cloacae complex Escherichia coli Klebsiella oxytoca Klebsiella pneumoniae Proteus Serratia marcescens

Gastrointestinal Panel Targets Bacteria Campylobacter (jejuni, coli and upsaliensis) Clostridium difficile (toxin A/B) Plesiomonas shigelloides Salmonella Yersinia enterocolitica Vibrio (parahaemolyticus, vulnificus and cholerae) Vibrio cholerae Diarrheagenic E. coli/Shigella Enteroaggregative E. coli (EAEC) Enteropathogenic E. coli (EPEC) Enterotoxigenic E. coli (ETEC) lt/st Shiga-like toxin-producing E. coli (STEC) stx1/stx2 E. coli O157 Shigella/Enteroinvasive E. coli (EIEC) Viruses Adenovirus F 40/41 Astrovirus Norovirus GI/GII Rotavirus A Sapovirus (I, II, IV and V) Parasites Cryptosporidium Cyclospora cayetanensis Entamoeba histolytica Giardia lamblia

Sample Requirements Respiratory Viral Panel Blood Culture ID Panel Nasal Wash Nasal Swab Sputum Respiratory specimen in Viral Transport Medium Gastrointestinal Panel Raw stool to be transferred to ETM Stool in ETM Blood Culture ID Panel Blood culture bottle that has been flagged positive by the Bacti Alert or Versatrek Analyzers

Positive Results Since Implementaion These are just a few… Limited exposure to potentially hazardous bacterial isolates in blood cultures Faster treatment and more guided treatment of patients at the point of entry and after admission Quick diagnosis Faster antimicrobial therapy implementation to speed recovery and reduce morbidity Admitting a patient versus not admitting a patient

Questions…