Advancing the state of the art in microscopy Imaging Protein Crystals and Managing the Associated Data.

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Presentation transcript:

advancing the state of the art in microscopy Imaging Protein Crystals and Managing the Associated Data

What Type of Microscope to Use?

–Sample Liquid Drop w/ Solid and/or Semi Solid

What Type of Microscope to Use? –Sample Liquid Drop w/ Solid and/or Semi Solid –Sample Location MicroBatch, Sitting Drop or Hanging Drop

Inverted Configuration Light Source Sitting Drop Or Microbatch Drop Microscope

Upright Configuration Microscope Hanging Drop Light Source

UprightInverted Sitting Drop Imaging Comparison

What Type of Microscope to Use? –Sample Liquid Drop w/ Solid and/or Semi Solid –Sample Location MicroBatch, Sitting Drop or Hanging Drop –Illumination Koehler

Properties of a Drop - Lens Effect

Properties of a Drop - Lens Effect - Side Wall/Refraction Ring

Less than 90º Contact Angle of a Drop

Hemispherical

Contact Angle of a Drop Greater than 90º

High Contrast = More Detail

Crystals at the Drop Edge - use medium contrast

Very dark side-wall effect - use low contrast

Focusing on condensation

Focusing on Crystals

Focusing on condensation Focusing on Crystals Low Contrast High Contrast

L.I.M.S. Laboratory Information Management System Data Management

Barcodes

Crystallization Data Experiment Details

Crystallization Data Experiment Details Image Management Scoring Images

Experiment Details Image Management Scoring Images Reviewing Results and Trends Crystallization Data

Experiment Details Image Management Scoring Images Reviewing Results and Trends Remote Viewing Crystallization Data

Experiment Details Image Management X-Ray Data

Experiment Details Image Management X-Ray Data Printing Results Crystallization Data

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