The Discovery of Introns The Nobel Prize in Physiology or Medicine 1993 "for their discoveries of split genes" Presented by 李柏毅 Institute of Bioinformatics,

Slides:



Advertisements
Similar presentations
Protein Synthesis. E. coli Ribosome -70S particle, MW ~2.5 x dissociable into small (30S) and large (50S) subunits -30S contains 16S RNA, 21 polypeptides.
Advertisements

Introduction to genomes & genome browsers
SBI 4U November 14 th, What is the central dogma? 2. Where does translation occur in the cell? 3. Where does transcription occur in the cell?
Revisions to the “central dogma” of molecular biology over the last 10 years, scientists have discovered an entirely new category of non-coding RNA genes.
© 2006 W.W. Norton & Company, Inc. DISCOVER BIOLOGY 3/e
Introduction to Virology. Nature of Viruses Viral genome is packaged in protein coat.
What is SV40 T-antigen? What does it do? Presented by 李柏毅 Genome Engineering Laboratory, Institute of Bioinformatics, National Yang-Ming University 2003/12/12.
DNA Replication When a cell or organism reproduces, a complete set of genetic instructions must pass from one generation to the next.
An Amazing Sequence Arrangement at the 5’ Ends of Adenovirus 2 Messenger RNA Louise T. Chow, Richard E. Gelinas, Thomas R. Broker and Richard J. Roberts.
Lecture 16: Processing of viral pre-mRNA
Chapter 6 Gene Prediction: Finding Genes in the Human Genome.
Eukaryotic cells modify RNA after transcription
AP Biology Chapter 15. Mutations AP Biology Code is redundant  several codons for each amino acid  “wobble” in the tRNA  “wobble”
Eukaryotic Gene Regulation
8.4 Transcription KEY CONCEPT Transcription converts a gene into a single-stranded RNA molecule.
8.6 Gene Expression and Regulation TEKS 5C, 6C, 6D, 6E KEY CONCEPT Gene expression is carefully regulated in both prokaryotic and eukaryotic cells.
Review of Protein Synthesis. Fig TRANSCRIPTION TRANSLATION DNA mRNA Ribosome Polypeptide (a) Bacterial cell Nuclear envelope TRANSCRIPTION RNA PROCESSING.
Dr. Kathleen Hill Assistant Professor Department of Biology The University of Western Ontario Office Hours: Monday 1 to 5pm Room 333 Western.
Chapters 26 Lehninger 5th Edition
Problem 1 Mutation in noncoding sequence change the number of protein molecules produce, but, generally each protein molecule made will have a normal amino.
BME 130 – Genomes Lecture 20 Gene expression and mRNA processing.
GenePolypeptide Gene  Polypeptide Transcription 1.RNAP binds to promoter 2.Separates DNA strands 3.Transcribes the DNA (adds RNA nucleotides in a 5'-3'
Prokaryotic cells turn genes on and off by controlling transcription.
Lecture 4 Topic 2. Gene Function & Gene Expression.
Topics in Bioinformatics CS832b Bin Ma. Lecture 1: Basic.
David Sadava H. Craig Heller Gordon H. Orians William K. Purves David M. Hillis Biologia.blu B – Le basi molecolari della vita e dell’evoluzione The Eukaryotic.
DNA LIBRARIES Dr. E. What Are DNA Libraries? A DNA library is a collection of DNA fragments that have been cloned into a plasmid and the plasmid is transformed.
Chapter 3 The Interrupted Gene.
Gene Regulation In 1961, Francois Jacob and Jacques Monod proposed the operon model for the control of gene expression in bacteria. An operon consists.
Functions of RNA mRNA (messenger)- instructions protein
Eukaryotic Genomes 11 November, 2005 Text Chapter 19.
Mutations Learning Goal: Identify mutations in DNA (point mutation and frameshift mutation caused by insertion or deletion) and explain how they can affect.
A Molecular Toolkit AP Biology Fall The Scissors: Restriction Enzymes  Bacteria possess restriction enzymes whose usual function is to cut apart.
Protein Synthesis “From code into Flesh & Blood”.
Introduction to Molecular Biology and Genomics BMI/CS 776 Mark Craven January 2002.
CFE Higher Biology DNA and the Genome Transcription.
AP Biology Chapter 17. RNA Processing AP Biology Transcription -- another look The process of transcription includes many points.
The Central Dogma of Molecular Biology DNA  RNA  Protein  Trait.
DNA TranscriptionTranslation The Central Dogma TraitRNA Protein Molecular Genetics - From DNA to Trait RNA processing.
Faculdade de Medicina da Universidade de Coimbra Curso de Medicina 1º Ano Ano lectivo 2009/2010.
Gene Expression & Regulation Chapter 8.6. KEY CONCEPT Gene expression is carefully regulated in both prokaryotic and eukaryotic cells.
DNA Technology. I. Vectors: Things used to transport genes into cells.
Reverse Transcription. POINT > Describe the process of reverse transcription POINT > Discuss the role of reverse transcription in retroviral infection.
Proteins Transcription ProcessingTranslationViruses.
Molecular Genetics Transcription. the process where the DNA code is used to make a complementary mRNA strand three main stages: 1. initiation 2. elongation.
Fig Prokaryotes and Eukaryotes
Chapter 17. Mutations
Transcription.
Prokaryotic cells turn genes on and off by controlling transcription.
Dr. Stuart Reichler Bio 325.
Prokaryotic cells turn genes on and off by controlling transcription.
The Operon Hypothesis The Operon Hypothesis was developed by 2 researchers: Jacob and Monod It explains how genes are regulated in prokaryotes. They received.
In eucaryotes, most genes are interrupted: the coding
Chapter 4 The Interrupted Gene.
Daily Warm-Up Dec. 11th -What are the three enzymes involved with replication? What is the function of each? Homework: -Read 13.1 Turn in: -Nothing.
Prokaryotic cells turn genes on and off by controlling transcription.
Gene Expression Activation of a gene to transcribe DNA into RNA.
Unit 7: Molecular Genetics
Prokaryotic cells turn genes on and off by controlling transcription.
Alternative RNA Splicing
From gene to protein.
Prokaryotic cells turn genes on and off by controlling transcription.
Prokaryotic cells turn genes on and off by controlling transcription.
Gene Structure.
VENKATRAMAN RAMAKRISHNAN ENGLAND THOMAS STEITZ USA ADA YONATH ISRAEL
Prokaryotic cells turn genes on and off by controlling transcription.
Introduction to Virology
Gene Structure.
Presentation transcript:

The Discovery of Introns The Nobel Prize in Physiology or Medicine 1993 "for their discoveries of split genes" Presented by 李柏毅 Institute of Bioinformatics, National Yang-Ming University

Background 周芷, Roberts and Sharp discovered that genes could be discontinuous in 1977 Richard J. RobertsPhillip A. Sharp New England Biolabs Beverly, MA, USA Massachusetts Institute of Technology (MIT), Center for Cancer Research Cambridge, MA, USA

Background During the first decades, studies on simple organisms, in particular bacteria and bacterial viruses.

Aim of Study To determine where in the genome different genes were located in adenovirus. This virus infects the cells of higher organisms, and its genome has many properties resembling those of the host cell.

The Experiment (cont.) …….ACCTGGCCC…… …….TGGAGCGGG…… transcription The two strands of DNA are separated DNA of adenovirus hybrid …….ACCTGGCCC…… …….TGGAGCGGG…… + mRNA

The Experiment (cont.)

In Eukaryotes

Alternative promoter selection Alternative tail site selection Alternative splicing by exon cassette selection Trans-Splicing Alternative Splicing EXON1 Intron1 EXON2 Intrin2 EXON3 P1P2T1T2 EXON1 Intron1 EXON2 Intrin2 EXON3 EXON1 Intron1 EXON2 Intrin2 EXON3

Discussion The most studied of such diseases is beta-thalassemia. The protein, beta-globin, forms part of hemoglobin. The life-span of the red blood cells is shortened resulting in anemia.

Discussion If the globin gene is damaged:

References Nobel e-Museum h.html tm David P. Clark, Lonnie D. Russell. Molecular Biology – made simple and fun / 2e (p ) Benjamin Lewin. GENES VI (p )

THANKS!