Selective and Differential Media

Slides:

Advertisements

Similar presentations

EMB agar Only gram-negative bacteria grow on EMB agar. (Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar.) Based.

Advertisements

Summary of Biochemical Tests in Microbiology

Quality Control of Culture media

IDENTIFIKASI BAKTERI OLEHSUDRAJAT FMIPA UNMUL 2009.

Differential medium. Differential medium helps us to differentiate one group of bacteria from another. Blood agar – differentiate hemolytic bacteria from.

Micro labs - review BIOL260 Winter Ubiquity What organisms grow best at room temperature? ___°C? At body temperature? = ___°C? What kind of medium.

Introduction In addition to general-purpose media, which allow the growth of most types of bacteria, microbiologists use specialized media to identify.

Lab equipment & procedures Chapter Ⅱ. Microscopes A. Light Microscopes 1. Single Lens 2. Compound microscopes eye pieceobjectiveTotal M Low P10X10X100X.

Ph. D Abdelraouf A. Elmanama

Microbial Growth Chapter 6.

Lab. No. 7. II. Enterobacteriaceae It divided into two main groups: It divided into two main groups: According to their effect on lactose  Lactose.

Media Preparation & Sterilization

Culture Media (Types, Preparation & Sterilization)

Culture Media Lab 2:.

CULTURE MEDIA LECTURE 5: Microbiology and Virology; 3 Credit hours

Media Preparation & Sterilization

Isolation of Staph, Strep and Gram – pathogen part II LAB # 13 Medgar Evers College Prof. Santos.

Selective and Differential media

Selective media Lab 6.

SSterile petri dish containing agar and usually nutrients UUsed to culture microorganisms.

Sample Comprehensive Laboratory exam

CULTURE MEDIA LECTURE 7: Microbiology and Virology; 3 Credit hours

Review of Gram Stain Selective and differential Media

Selected and Differential Media. Define Selective Media Differential Media Enriched Media.

SELECTIVE, DIFFERENTIAL AND ENRICHED MEDIA

Lab 13- Bacterial cultivation

Differential and Selective Media Prepared BY Ms. c: Abed Al Rahman I

Gram-negative rods Enterobacteriaceae.

Biochemical Characterization

Lab 2: Culture Media. In this lab we learn about different types of media that are used to grow bacteria. Some types of media will grow just about any.

Ex. 13: Urine Culture Technique and the Importance of Selective and Differential Media for Gram-Negative Rods Objectives ??

Microbial Control Lab 4. Selective and Differential Media We have completed Isolation of bacteria using steak plate and spread plate This is a good beginning,

MICROSCOPES. Microscopes Microscope : an instruments used to examine very small objects (Specimens) in microbiology. Functions : To allow us to study.

Gram Positive Staphylococci – (Note the purple, spherically- shaped, clustered cells)

University of Tabuk Faculty of Applied Medical Science

Isolation and identification of Enteric Bacteria

Lab. No. 5. Gram-negative, non-spore-forming bacilli. Gram-negative, non-spore-forming bacilli. Their natural habitat is the intestinal tract of humans.

Isolation of Staphylococcus aureus and Streptococcus

PHT 416 Lab 8. Steps Microscopic Morphology Growth Biochemical Tests Nutrient agar Blood agar Mannitol Salt Agar MacConkey’s agar.

Lab. No. 4 (A). StaphylococciStreptococciMicrococci NeisseriaCorynbacterium Clostridum Bacillus Enterobacteriaceae Pseudomonas. Bacteria Gram’s Stain.

Exercise 41: Multiple Test Media: Read and record results

Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.

Components Preparation Inoculation

Chapter 6 Microbial Nutrition and Growth. Microbial Growth Microorganisms are found in the harshest of environments – Deep ocean – Volcanic vents – Polar.

Selective and differential Media By : Mahmoud W. El-Hindi

GRAM STAINING. OBJECTIVES ● Describe reagents used in Gram stain & purpose of these reagents. ● Color expected of Gram Pos & Gram Neg after performing.

Types of culture media categorized according to their function and use . In diagnostic microbiology there are four general categories of media. 1.Enrichment.

Gram-negative rods Enterobacteriacea Clinical Microbiology

Selective and Differential Media

culturing of microorganism

Mystery Growth Laboratory Practical

Selective and Differential Media

Microbiology Lab # 11 Medgar Evers College Dr. Santos

PENGENALAN KULTUR MEDIA BAKTERI

Cultivation of Bacteria

Week 5 B Exp 10: Gram Stain Gram Stain: Gram + SE, SA

Selective and differential Media By : Mahmoud W. El-Hindi

Urine Culture Technique and the Importance of Selective and Differential Media for Gram-Negative Rods Vocabulary Coliforms Pure culture Types of culture.

Culture Media Lab 2:.

Urine Culture Technique and the Importance of Selective and Differential Media for Gram-Negative Rods Day 2 Remember this plate?

Introduction In addition to general-purpose media, which allow the growth of most types of bacteria, microbiologists use specialized media to identify.

Media Preparation & Sterilization

Introduction In addition to general-purpose media, which allow the growth of most types of bacteria, microbiologists use specialized media to identify.

Presentation transcript:

Selective and Differential Media Selective media Media designed to inhibit the growth of some organisms while encouraging the growth of others Differential media Media that contains substances (indicators) that reveal differences between organisms Media can be both selective and differential.

Mannitol Salt agar Both a selective and a differential medium

PEA Phenylethyl Alcohol Agar A selective media that inhibits the growth of most Gram negative microbes

Blood agar

EMB Eosin Methylene Blue Both a selective and a differential medium. Dyes inhibit Gram positives

HEA (Hektoen Enteric Agar) Both a selective and a differential medium. Bile salts inhibit Gram positives HEA (Hektoen Enteric Agar)

Hektoen enteric (HE) agar. HE Agar Plates Hektoen enteric (HE) agar. Salmonella produces greenish colonies with black centers as shown in the left panel. Enterobacter produces bright orange colonies as shown in the right panel.

MacConkey’s Agar Both a selective and a differential medium. Bile salts and crystal violet inhibit Gram positive bacteria