Chemistry on Ultrafast and Ultrasmall Scales Trevor Smith Ken Ghiggino Paul Mulvaney School of Chemistry, University of Melbourne.

Slides:

Advertisements

Similar presentations

Advertisements

Components of ultrafast laser system

Suppose that you hold the transparency in the photograph below in front of a mirror. How will its reflection appear? Is the image: (1) inverted top-to-bottom?

Prashant V. Kamat Radiation Laboratory, University of Notre Dame, Notre Dame, Indiana

Fluorophores bound to the specimen surface and those in the surrounding medium exist in an equilibrium state. When these molecules are excited and detected.

R. Hui Photonics for bio-imaging and bio- sensing Rongqing Hui Dept. Electrical Engineering & Computer Science, The University of Kansas, Lawrence Kansas.

Basics of femtosecond laser spectroscopy

New high-power ultrafast laser and potential applications in biology and medicine Jeremy Allam Optoelectronic Devices and Materials Research Group Tel.

DNA Photonics Hieu Bui 20 September Outline Fluorescent resonance energy transfer (FRET) Fluorescent Labels – Fluorophores, quantum dots – Single.

Short pulses in optical microscopy Ivan Scheblykin, Chemical Physics, LU Outline: Introduction to traditional optical microscopy based on single photon.

Single Molecule Fluorescence from Organic Dyes in Thin Polymer Films Robin Smith and Carl Grossman, Swarthmore College March 5, 2003.

Confocal & Two-photon Microscopy. Contents 1.Two-Photon Microscopy : Basic principles and Architectures 2. Resolution and Contrast in Confocal and Two-Photon.

Network Challenges: l Increase usage/awareness of high tech equipment l Lack of continuity, post docs, etc. - long term l Aim to create a Centre (of Excellence?

Micro PIV  An optical diagnostic technique for microfluidics (e.g. MEMS, biological tissues, inkjet printer head) Requirements: Measure instantaneously.

Single Photon Source for Quantum Communication Sarah Walters, Meng-Chun Hsu, Hubert Zal, Pierce Morgan.

Microscopy is about a combination of resolution (seeing smaller and smaller things), and contrast (seeing what you want to see). Both aspects have recently.

Fiber Optics Defining Characteristics: Numerical Aperture Spectral Transmission Diameter.

Sub-diffraction-limit imaging by Stochastic Optical Reconstruction Microscopy (STORM) Michael J. Rust, Mark Bates, Xiaowei Zhuang Harvard University Published.

Microscopy is about a combination of resolution (seeing smaller and smaller things), and contrast (seeing what you want to see). Both aspects have recently.

Data Analysis Issues in Time-Resolved Fluorescence Anisotropy Bill Laws Department of Chemistry University of Montana Support from NSF EPSCoR, NIH, and.

Pump-Probe Spectroscopy Chelsey Dorow Physics 211a.

Międzyresortowy Instytut Techniki Radiacyjnej Prof. dr hab. Halina Abramczyk Dr hab. inż. Beata Brożek-Płuska Dr inż. Jakub Surmacki Mgr inż. Monika Kopeć.

IBPhotonics Ltd. Company Advantages IBPhotonics Ltd. Light Instruments for a Bright Future  Strong team with 20+ years of engineering and scientific background.

LASERs Light Amplification by Stimulated Emission of Radiation.

4-1 Chap. 7 (Optical Instruments), Chap. 8 (Optical Atomic Spectroscopy) General design of optical instruments Sources of radiation Selection of wavelength.

Time-Correlated Single Photon Counting (TCSPC) Scott Thalman Brigham Young University Advisor: Dr. John Colton Dr Haeyeon Yang USU Physics Help from Mitch.

Energy Transfer in a Light Harvesting Dendron Lea Nienhaus ISMS06/17/14.

Common types of spectroscopy

Ultrafast Experiments Hangwen Guo Solid State II Department of Physics & Astronomy, The University of Tennessee.

Properties of ElectroMagnetic Radiation (Light)

Illumination and Filters Foundations of Microscopy Series Amanda Combs Advanced Instrumentation and Physics.

1 Femtosecond Time and Angle-Resolved Photoelectron Spectroscopy of Aqueous Solutions Toshinori Suzuki Kyoto University photoelectron.

Femtosecond low-energy electron diffraction and imaging

TIRF Total Internal Reflection Fluorescence Microscopy specialized fluorescence microscopy technique specifically images a very thin optical section (50-250nm)

Ultrafast Spectroscopy Of Methyl Viologen: Effects Of Zeolite Entrapment Joseph Henrich, Haoyu Zhang, Jeremy White, Prabir Dutta and Bern Kohler The Ohio.

Adding Polarimetric Multiphoton Imaging to the W. M. Keck 3-Dimensional Fusion Microscope Objective4x/0.2 Air10x/0.45 Air20x/0.75 Air40x/0.80 Water60x/1.45.

ULTRA-BROAD BANDWIDTH CAVITY ENHANCED ABSORPTION SPECTROSCOPY Paul S. Johnston Kevin K. Lehmann Department of Chemistry University of Virginia.

IPC Friedrich-Schiller-Universität Jena 1 7. Fluorescence microscopy 7.3 FRET microscopy - The different Dipoles 1. static electric Dipole: Far away: E.

Mikael Siltanen,1 Markus Metsälä,1

Automated 2D IR Spectroscopy using a high repetition rate laser system

Two-Focus Fluorescence Correlation　 Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements Jörg Enderlein et al., ChemPhysChem, 8, 433–443.

Faculty of Chemistry, Adam Mickiewicz University, Poznan, Poland 2012/ lecture 4 "Molecular Photochemistry - how to study mechanisms of photochemical.

Single Molecule Spectroscopy (SMS) 2010/6/9 Miyasaka Lab. Iida Atsushi.

Probing into the Molecular World with Light Jung Y. Huang Department of Photonics and Institute of Electro-Optical Engineering, NCTU.

Fluorescence Spectroscopy

Sowmya Vasa, Umar Alqasemi, Aditya Bhargava. Objectives This paper aims in bringing out a novel light microscopy method called Focal Modulation Microscopy.

Scanning excitation and emission spectra I Wavelength (nm) )Scan excitation with emission set at 380 nm -λ ex,max = 280 nm 2) Scan emission.

Fluorescence Correlation Spectroscopy

02/20/2015PHY 712 Spring Lecture 161 PHY 712 Electrodynamics 9-9:50 AM Olin 103 Plan for Lecture 16: Read Chapter 7 1.Plane polarized electromagnetic.

OptoPIC Ultrafast Gated Camera. OptoPIC Features ● MHz Rep. Rate ● 200 ps – 1 ns Gating ● Single-Photon Sensitivity ● Integrated Delay Generator.

The distribution of electrons of an atom or molecule (or other physical structure) in atomic or molecular orbitals an energy is associated with each electron.

Optics Focus Confocal theory.

Ultrafast techniques Laser systems Ti:Saph oscillator/regen, modelocking NOPA’s Pump-probe absorption difference spectroscopy Two-color Dispersed detection.

Properties of ElectroMagnetic Radiation (Light)

Powerpoint Templates Page 1 Powerpoint Templates Electronic Spectroscopy Time Resolved Fluorescence.

Near Field Scanning Optical Microscopy (NSOM, SNOM, NFOM) Stephanie Pruzinsky Group Meeting, June 6, 2002.

Department of Applied Chemistry Web Site:

Evanescent wave induced Time-resolved Fluorescence

Optics Kathy Geise April 2007

Warren Huey CHEM /29/17.

Principle of Mode Locking

Announcements 1/23/12 Prayer SPS social.

New Turf for CFP/YFP FRET Imaging of Membrane Signaling Molecules

New Turf for CFP/YFP FRET Imaging of Membrane Signaling Molecules

Scalar theory of diffraction

TIME RESOLVED SPECTROSCOPY [T.R.S.]:

FLUORESCENCE MICROSCOPY

Dynamics of Energy Transfer in Cylindrical Molecular Aggregates

Dynamics of Energy Transfer in Cylindrical Molecular Aggregates

“Small and Fast: femtosecond light-matter interactions at 0

Presentation transcript:

Chemistry on Ultrafast and Ultrasmall Scales Trevor Smith Ken Ghiggino Paul Mulvaney School of Chemistry, University of Melbourne

Ultrasmall - techniques l Confocal and multiphoton microscopy l Single molecule detection and emission spectroscopy l FCS, antibunching l Polymers & quantum dots l Total internal reflection fluorescence (EWIF)

Ultrasmall - facilities l Scanning confocal and multiphoton microscopy l Time-resolved fluorescence imaging  LaVision PicoStar  TCSPC (EI, Becker & Hickl, other) l Single molecule detection and emission spectroscopy l FCS, antibunching, single molecule spectroscopy (spectrograph/CCD) l Polymers & quantum dots l Total internal reflection fluorescence (EWIF) l Scanning near field (SNOM)

Ultrafast - techniques l Time-correlated single photon counting l Time-resolved fluorescence anisotropy l Flash photolysis l Pump-probe techniques l Time-resolved absorption l Fluorescence upconversion l Multi-pulse, multi-wavelength photon echoe

Ultrafast - facilities l Nanosecond flash photolysis (Nd:YAG/OPO) l ns gated CCD/spectrometer l Picosecond dye lasers l Femtosecond Ti:sapphire systems l oscillator/OPO system  Pulse picked/cavity dumped  MHz rep. rates, nJ pulse energies, tuneable l amplified femtosecond Ti:sapphire oscillator/OPA  <280 kHz rep. rates, µJ-10s of nJ pulse energies, tuneable

Facilities l Synthesis l Light emitting polymers (PPVs) l Quantum dots

Evanescent Waves n1n1 n2n2 n1> n2n1> n2  Standing wave Total internal reflection (if  i >  c ) Evanescent wave E=E o exp(-x/  ) Refraction (if  i <  c ) Incident Beam, E o Interface Normal ii

l EWIF spectroscopy l EWIF-FCS l Time-resolved EWIFS l Time-resolved EWIF anisotropy l Time-resolved EWIF microscopy

l Expressions for polarisations perpendicular, s, & parallel, p, to plane of incidence: l In bulk solution: l In-plane: l Out-of-plane: Polarised EWIF z x y

TREWIF Anisotropy Time (ns) BULK  = 64 nm  = 76 nm  = 113 nm INCREASING DISTANCE AWAY FROM INTERFACE r(t)

“In-plane” and “Out-of- Plane” anisotropy

Time-resolved EWIF microscopy l Resolution from interface ~10’s of nm