Advanced molecular genetics 224

Instructor e.Mail me to set an appointment Office, Botany Department Room #239 in the first floor

Course contents - The history of DNA discovery - Introduction to classic genetics - Introduction to molecular genetics - DNA structure and function - What is the gene? - Replication, transcription and translation processes in prokaryotes and eukaryotes. - RNA and protein - Gene expression - Molecular genetic techniques -Genetic engineering and DNA cloning

Course requirements Weekly in class quizzes Take home assignments ( a assignemnts) Midterm exam ( for lecture and lab) Final exam ( for lecture and lab) Oral presentation Attendance

Lab session -Biostatistics - Data presentation in your lab report - ( making graphs and tables)

Lab session -DNA Isolate DNA and determine its concentration. - Analysis of DNA using gel electrophoresis. - DNA amplification using PCR techniques - DNA sequencing ( classic and modern methods) -Restriction enzymes and restriction map.

Lab session Protein - Isolation and analysis - Electrophoresis and concentration determination - Western technique

Lab session Genetic engineering - DNA cloning and plasmid design - Transformation and competent cell preparation -

Lab session -Oral presentations

Introduction of some concepts

Micro and macro molecules Micro-molecules: Glucose, amino acids ….etc. Macro-molecules DNA, RNA and proteins

Isolation of macromolecules Break the cell open Break the cell open This process is called cell lysis This process is called cell lysis Cells must be placed in a buffer solution during the lysis process. why? Cells must be placed in a buffer solution during the lysis process. why?

Isolation of macromolecules

Hierarchy & Protein Function

Protein Folding II

Regulation of Protein Function by Degradation

electrophoresis

SDS-PAGE

Introduction to genetics

What is the genetic material

S and R bacterial cells Streprococcus pneumonia

Hershy – Chase “ blender ” experiment using T2 bacteriophage

Bacteriophage

How genes are transmitted from parents to offspring?

Classical or mendelian genetics Gregory Mendel experiment on Garden peas Gregory Mendel experiment on Garden peas

Classical genetics depends on: Phenotype: Phenotype: color, size, texture … etc. color, size, texture … etc. Genotype: Genotype: The genetic make up of the organism. The genetic make up of the organism.

Inheritance of seed texture Morphological phenotype W is dominant over the w

Inheritance of seed texture Molecular phenotype

Mendel first experiment Inheritance of Genetic trait(s)

Mendel first experiment reciprocal cross

Results of Mendel first experiment

Explanation of 3 dominant :1 recessive inheritance Monohybrid cross Monohybrid cross Independent assortment Independent assortment Segregation Segregation

The principal of the 3:1 inheritance Phenotype: Genotype: