Protocol for ELISA Sample Submission-384 well: General Procedures Primary assays: x-40 & x-42. (1-40, 1-42, & 1-total on special request). Linear range:

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Research Techniques Made Simple: Enzyme Immunoassay and ELISA

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Protocol for ELISA Sample Submission-384 well: General Procedures Primary assays: x-40 & x-42. (1-40, 1-42, & 1-total on special request). Linear range: 1pM (4pg/ml) - 110pM (500pg/ml). 50uL/well is needed for each assay. If both x-40 and x-42, submit 90uL. 2G3 and 21F12 capture antibodies used for x-40 and x-42 assays, respectively; 266B antibody used for detection. Submit samples on 96 well plate!! Please DO NOT use tissue culture plates. Please leave 1 row empty for peptide standard, and 1 column empty. Always submit a submission sheet ahead of time. Even if you talk to Ting about preparing a submission, you should submit a sheet at that time. This is the ONLY way that Ting can plan ahead and prepare for assays. Submission sheets can be ed to Ting or dropped off at Ting’s desk (Harvard Institute of Medicine, Xia Lab, HIM 610).

Your sample layout (on 96 well plate): --For 1 assay submit 50ul/sample; for 2 assays(x-40 & x-42) submit 90ul/well --Add samples to a 96 well plate, starting at B1 and moving across, as shown on left below. --Fill all 11 wells in each row before starting another row. --Fill up to 7 rows. Protocol for ELISA Sample Submission-duplicate: sample layout ABCDEFGHABCDEFGH

Example of sample submission sheet 384 well duplicate