Compound Light Microscopes

Parts Identification

Lenses Eyepiece – 10X Eyepiece – 10X Objective Lenses Objective Lenses –Low power – 4X * 10X = 40X red –Medium power – 10X *10X = 100X yellow –High power – 40X * 10X = 400X blue

Proper Handling Carry by the base and arm – two hands! Carry by the base and arm – two hands! Set down gently Set down gently Do not touch lenses Do not touch lenses

Focusing Adjust light with dial Adjust light with dial –1 = lowest light 5 = highest light Focus first with low power and coarse adjustment Focus first with low power and coarse adjustment Then focus at medium power with fine adjustment Then focus at medium power with fine adjustment Last focus at high power with fine adjustment Last focus at high power with fine adjustment Use of coarse adjustment at medium or high power will result in an “F” Use of coarse adjustment at medium or high power will result in an “F”

Drawing Field of View 3. Description of specimen 4. Magnification 1. Draw circle for field of view 2. Draw specimen to scale

Clean Up Low power objective Low power objective Eyepiece over arm Eyepiece over arm Wrap cord neatly around microscope Wrap cord neatly around microscope Rinse and dry slides and coverslips Rinse and dry slides and coverslips Nobody leaves until all microscopes are properly cleaned up Nobody leaves until all microscopes are properly cleaned up