What is the growing mechanism of filamentous fungi, such as N. crassa? Project by Thomas Lew Mentor: Dr. Michael Freitag.

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Presentation transcript:

What is the growing mechanism of filamentous fungi, such as N. crassa? Project by Thomas Lew Mentor: Dr. Michael Freitag

Why this important? Advances knowledge of fungal biology Could lead to a way to stop growth of infectious fungi (i.e. selective targeting)

Examples of Infectious Fungal Diseases Aspergillosis Fungal pneumonia

Why N. Crassa? One of the oldest genetic model Filamentous Easier to manipulate

What we think happens: Microtubule Organization Center (at nuclei) Apical Growth Body

Methods PCR – subcloning and N. crassa transformation Replace Mal3 MT end binding protein with GFP-tagged Mal3 MT Replace Tip1 MT binding protein with GFP-tagged Tip1 MT Microscopy Track growth and direction

Polymerase Chain Reaction (PCR)

GFP

Results

Acknowledgements Howard Hughes Medical Institute Dr. Kevin Ahern Dr. Michael Freitag Dr. Michael Gross Grant Farr All the wonderful people at OSU that helped us out